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Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Szabados, LMroginski, LARoca, William M.[Suspensiones celulares: descripción, manipulación y aplicaciones]Suspensiones celulares: descripción, manipulación y aplicaciones
Escobar Pérez, Roosevelt H.Santos Meléndez, Luis GuillermoTohme, Joseph M.Roca, William M.[Cryopreservation of FEC lines as an aid for genetic transformation programs]Cryopreservation of FEC lines as an aid for genetic transformation programs
Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaDebouck, Daniel G.Tohme, Joseph M.Roca, William M.[Cryopreservation of cassava shoot tips using the encapsulation-dehydration technique]Cryopreservation of cassava shoot tips using the encapsulation-dehydration technique

Cassava is mostly growing by resource-limited farmers in the Americas, Africa, Asia and Oceania. The crop is an important source of food and income for millions in the tropics. Vegetative propagation, bulkiness of planting material and risk of genetic erosion make cassava an ideal target for inno...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[Cryopreservation for long-term conservation of cassava genetic resources]Cryopreservation for long-term conservation of cassava genetic resources

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