A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported pre...
Hussain, A.
,
Espinel, MT
,
Roca, William M.
,
Bushhuk, W
,
[Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)]
,
Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)
Hussain, A.
,
Bushuk, W
,
Ramírez, H.
,
Roca, William M.
,
[Polyacrylamide gel electrophoresis procedures for cultivar identification of field bean, cassava and pasture legumes = Procedimientos de electroforesis en geles de poliacrilamida para la identificación de cultivares de frijol, yuca y leguminosas forrajeras]
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Polyacrylamide gel electrophoresis procedures for cultivar identification of field bean, cassava and pasture legumes = Procedimientos de electroforesis en geles de poliacrilamida para la identificación de cultivares de frijol, yuca y leguminosas forrajeras
For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro ...
Roca, William M.
,
Chavez, R.
,
Martin, M.L..
,
Arias, D.I.
,
Mafla, G.
,
Reyes, Raúl
,
[In vitro methods of germ-plasm conservation]
,
In vitro methods of germ-plasm conservation
Ramírez, H.
,
Calderón, A.
,
Roca, William M.
,
[Técnicas moleculares para evaluar y mejorar el germoplasma vegetal]
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Técnicas moleculares para evaluar y mejorar el germoplasma vegetal
Hussain, A.
,
Ramírez, H.
,
Roca, William M.
,
[Manual práctico para la detección electroforética de isoenzimas y otras proteínas]
,
Manual práctico para la detección electroforética de isoenzimas y otras proteínas
Several important aspects of the use of molecular markers in cassava germplasm characterization and in plant breeding programs are indicated. Basic concepts considered include protein and DNA electrophoresis. In the case of cassava, isoenzyme electrophoresis was applied to confirm duplicates dete...
Ramírez, H.
,
Calderón, A.
,
Roca, William M.
,
[Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal]
,
Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal
Angel, F.
,
Arias Garzón, D.I.
,
Roca, William M.
,
[DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR]
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DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR