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Different aspects of anther culture are reviewed as follows: its application in plant breeding, conditions that affect results (physiology and genotype of donor plants, albinism, pollen development, pretreatment of anthers, culture media), var. obtained by anther culture, and comparative advantag...


Roca, William M.Nunez, VMMornan, K[Cultivo de anteras y mejoramiento de plantas]Cultivo de anteras y mejoramiento de plantas

Several important aspects of the use of molecular markers in cassava germplasm characterization and in plant breeding programs are indicated. Basic concepts considered include protein and DNA electrophoresis. In the case of cassava, isoenzyme electrophoresis was applied to confirm duplicates dete...


Ramírez, H.Calderón, A.Roca, William M.[Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal]Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...


Roca, William M.Nolt, Barry L.Mafla, G.Roa E, J.C.Reyes, R.[Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)]Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)

In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2...


Roca, William M.Arias, D.I.Chavez, R.[Metodos de conservacion in vitro del germoplasma]Metodos de conservacion in vitro del germoplasma

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