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Conference Paper 1995

Autor: Escobar Pérez, Roosevelt H. Mafla, G. Roca, William M.

Cassava is mostly growing by resource-limited farmers in the Americas, Africa, Asia and Oceania. The crop is an important source of food and income for millions in the tropics. Vegetative propagation, bulkiness of planting material and risk of genetic erosion make cassava an ideal target for inno...

Enlace original: https://cgspace.cgiar.org/handle/10568/55848

Investigación en Agricultura
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Escobar Pérez, Roosevelt H. ,  Mafla, G. ,  Roca, William M. ,  [Cryopreservation for long-term conservation of cassava genetic resources] ,  Cryopreservation for long-term conservation of cassava genetic resources
Conference Paper 1995

Autor: Sarria, RA Balcázar, N Destéfano Beltrán, L.J.C. Roca, William M.

To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains th...

Enlace original: https://cgspace.cgiar.org/handle/10568/55850

Investigación en Agricultura
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Sarria, RA ,  Balcázar, N ,  Destéfano Beltrán, L.J.C. ,  Roca, William M. ,  [Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)] ,  Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)
Conference Paper 1995

Autor: Gómez, R. Angel, F. Bonierbale, Merideth W. Rodríguez, F. Tohme, Joseph M. Roca, William M.

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...

Enlace original: https://cgspace.cgiar.org/handle/10568/55846

Investigación en Agricultura
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Gómez, R. ,  Angel, F. ,  Bonierbale, Merideth W. ,  Rodríguez, F. ,  Tohme, Joseph M. ,  Roca, William M. ,  [Selecting heterozygous parents and single-dose markers for genetic mapping in cassava] ,  Selecting heterozygous parents and single-dose markers for genetic mapping in cassava
Conference Paper 1995

Autor: Henshaw, GG Roca, William M.
Enlace original: https://cgspace.cgiar.org/handle/10568/55855

Investigación en Agricultura
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Henshaw, GG ,  Roca, William M. ,  [Report of the working group on Manihot germplasm conservation, exchange and micropropagation] ,  Report of the working group on Manihot germplasm conservation, exchange and micropropagation
Conference Paper 1993

Autor: Sarria, RA Gómez, A. Cataño C, ML Herrera, P.V. Calderón, A. Mayer, Jorge Edgard Roca, William M.

Our strategy for Aqrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Aqrobacterium strains as well as regeneration protocols have been developed; the plasmid ...

Enlace original: https://cgspace.cgiar.org/handle/10568/55690

Investigación en Agricultura
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Sarria, RA ,  Gómez, A. ,  Cataño C, ML ,  Herrera, P.V. ,  Calderón, A. ,  Mayer, Jorge Edgard ,  Roca, William M. ,  [Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation] ,  Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation
Conference Paper 1993

Autor: Cataño C, ML Mornan, K Plazas, J Roca, William M.

Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pol...

Enlace original: https://cgspace.cgiar.org/handle/10568/55689

Investigación en Agricultura
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Cataño C, ML ,  Mornan, K ,  Plazas, J ,  Roca, William M. ,  [Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos] ,  Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos
Conference Paper 1993

Autor: Mafla, G. Roca, William M. Reyes, R. Roa E, J.C. Muñoz M, L. Baca, AE Iwanaga, Masaru
Enlace original: https://cgspace.cgiar.org/handle/10568/55702

Investigación en Agricultura
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Mafla, G. ,  Roca, William M. ,  Reyes, R. ,  Roa E, J.C. ,  Muñoz M, L. ,  Baca, AE ,  Iwanaga, Masaru ,  [In vitro management of cassava germplasm at CIAT] ,  In vitro management of cassava germplasm at CIAT
Conference Paper 1993

Autor: Sarria, RA Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, William M.

Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were...

Enlace original: https://cgspace.cgiar.org/handle/10568/55686

Investigación en Agricultura
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Sarria, RA ,  Ocampo Nahar, César H. ,  Ramírez, H. ,  Hershey, Clair H. ,  Roca, William M. ,  [Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz)] ,  Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz)
Conference Paper 1993

Autor: Angel, F. Giraldo, F. Gómez, R. Iglesias, C.A. Tohme, Joseph M. Roca, William M.

The construction of a detailed genetic map of cassava (Manihot esculenta Crantz) will be useful to tag agronomically important trais as well as to isolate and to clone cassava genes in the future. This map will also contribute to the understanding of cassava genomic structure and evolution. As a ...

Enlace original: https://cgspace.cgiar.org/handle/10568/55685

Investigación en Agricultura
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Angel, F. ,  Giraldo, F. ,  Gómez, R. ,  Iglesias, C.A. ,  Tohme, Joseph M. ,  Roca, William M. ,  [Use of RFLPs and RAPDs in cassava genomic studies] ,  Use of RFLPs and RAPDs in cassava genomic studies

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