Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...
Escobar Pérez, Roosevelt H.
,
Mafla, G.
,
Roca, William M.
,
[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]
,
A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were ...
Marin, M.L.
,
Mafla, G.
,
Roca, William M.
,
Withers, Lyndsey A.
,
[Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen]
,
Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen
Fregene, Martin A.
,
Ospina, Julio A.
,
Roca, William M.
,
[Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos]
,
Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos
Thro, Ann Marie
,
Roca, William M.
,
Restrepo, J.
,
Caballero, H.
,
Poats, Susan V.
,
Escobar Pérez, Roosevelt H.
,
Mafla, G.
,
Hernández, C.
,
[Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America]
,
Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America
La unidad de recursos genéticos y el programa de yuca de CIAT han iniciado un proyecto conjunto de investigación sobre cultivos de meristemos con el fin de conseguir facilitar la conservación y el intercambio de germoplasma de Manihot
Roca, William M.
,
[La nueva biotecnología de plantas: Aplicaciones en la agricultura]
,
La nueva biotecnología de plantas: Aplicaciones en la agricultura
Muñoz Flórez, Ligia Carmenza
,
Blair, Matthew W.
,
Duque E., Myriam Cristina
,
Roca, William M.
,
Tohme, Joseph M.
,
[Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines]
,
Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines
Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...
Sánchez, I.
,
Angel, F.
,
Grum, M.
,
Duque E., Myriam Cristina
,
Lobo Arias, M
,
Tohme, Joseph M.
,
Roca, William M.
,
[Variability of chloroplast DNA in the genus Passiflora L.]
,
Variability of chloroplast DNA in the genus Passiflora L.
Los trabajos realizados en varias especies de plantas revelan que una porción significativa de los genes que se expresan en el esporofito también lo hacen en el grano de polen, en consecuencia la presión de selección a nivel de polen debe conducir a modificaciones en caracteres cualitativos o cu...
Vásquez, L.M.
,
Montes Rojas, C
,
White, Jeffrey W.
,
Roca, William M.
,
[Metodología para la selección de líneas de fríjol Phaseolus vulgaris L. por estrés de temperatura a nivel microgametofítico]
,
Metodología para la selección de líneas de fríjol Phaseolus vulgaris L. por estrés de temperatura a nivel microgametofítico