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Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen

Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were ...


Marin, M.L.Mafla, G.Roca, William M.Withers, Lyndsey A.[Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen]Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen
Fregene, Martin A.Ospina, Julio A.Roca, William M.[Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos]Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos
Thro, Ann MarieRoca, William M.Restrepo, J.Caballero, H.Poats, Susan V.Escobar Pérez, Roosevelt H.Mafla, G.Hernández, C.[Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America]Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America

La unidad de recursos genéticos y el programa de yuca de CIAT han iniciado un proyecto conjunto de investigación sobre cultivos de meristemos con el fin de conseguir facilitar la conservación y el intercambio de germoplasma de Manihot


Roca, William M.[Tissue culture at CIAT]Tissue culture at CIAT
Muñoz Flórez, Ligia CarmenzaBlair, Matthew W.Duque E., Myriam CristinaRoca, William M.Tohme, Joseph M.[Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines]Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines

Roca, William M.Mroginski, LA[Cultivo de tejidos en la agricultura: fundamentos y aplicaciones]Cultivo de tejidos en la agricultura: fundamentos y aplicaciones

Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...


Sánchez, I.Angel, F.Grum, M.Duque E., Myriam CristinaLobo Arias, MTohme, Joseph M.Roca, William M.[Variability of chloroplast DNA in the genus Passiflora L.]Variability of chloroplast DNA in the genus Passiflora L.

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