The lack of good quality planting material of farmers' cassava varieties, produced locally and at low cost, is a major constraint limiting the expansion of cassava production in Colombia. This article describes the adaptation of conventional cassava propagation to a low-input scheme for rural tis...
Escobar Pérez, Roosevelt H.
,
Hernández, C.M.
,
Larrahondo, N
,
Ospina, GI
,
Restrepo M., José M.
,
Muñoz Flórez, Ligia Carmenza
,
Tohme, Joseph M.
,
Roca, William M.
,
[Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia]
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Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia
Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...
Sarria, RA
,
Torres Castro, Edgar Alonso
,
Angel, F.
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
[Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation]
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Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation
Estado actual del mejoramiento del arroz mediante la utilización de especies silvestres de arroz en CIAT. Este trabajo presenta el uso de especies silvestres de arroz en la obtención de germoplasma mejorado con mayor potencial de rendimiento y una base genética ampliada. Tres especies silvestres ...
Martínez Racines, César Pompilio
,
Tohme, Joseph M.
,
López Gerena, J.
,
Borrero Correa, Jaime C.
,
McCouch, Susan R.
,
Roca, William M.
,
Chatel, M
,
Guimaraes, Elcio Perpétuo
,
[Estado actual del mejoramiento del arroz mediante la utilización de especies silvestres de arroz en CIAT]
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Estado actual del mejoramiento del arroz mediante la utilización de especies silvestres de arroz en CIAT
Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Ramírez, H.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
Tohme, Joseph M.
,
López, Y.
,
[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético
Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...
Fajardo, Diego
,
Angel, F.
,
Grum, M.
,
Tohme, Joseph M.
,
Lobo Arias, M
,
Roca, William M.
,
Sánchez, I.
,
[Genetic variation analysis of the genus Passiflora L. using RAPD markers]
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Genetic variation analysis of the genus Passiflora L. using RAPD markers
Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...
Fregene, Martin A.
,
Okogbenin, Emmanuel
,
Mba, C
,
Angel, F.
,
Suarez, MC
,
Gutiérrez A., Janeth Patricia
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Bonierbale, Merideth W.
,
Tohme, Joseph M.
,
[Genome mapping in cassava improvement: Challenges, achievements and opportunities]
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Genome mapping in cassava improvement: Challenges, achievements and opportunities
A polyacrylamide gel electrophoresis procedure was developed for discriminating cultivars of the forage legume Pueraria phaseoloides on the basis of the patterns of cotyledon proteins. The genotypic marker proteins were extracted with 5M acetic acid and electrophoresed at pH 3.1 in aluminum lacta...
Hussain, A.
,
Bushuk, W
,
Roca, William M.
,
[Identification of cultivars of the forage legume Pueraria phaseoloides by electrophoretic patterns of storage proteins]
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Identification of cultivars of the forage legume Pueraria phaseoloides by electrophoretic patterns of storage proteins
Cassava (Manihot esculenta Crantz) is a tough and productive survival crop of smallholder farmers in the tropics. It is often grown where other crops fail. Research can make important contribution to food security and economic development in cassava-growing areas. This can be enhanced through in-...
Thro, Ann Marie
,
Roca, William M.
,
Iglesias, C.A.
,
Henry, Guy
,
Ng, S.Y.C.
,
[Contributions of in-vitro biology to cassava improvement]
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Contributions of in-vitro biology to cassava improvement
Congruity and recurrent backcross interspecific hybrids between common bean (Phaseolus vulgaris L.) and tepary bean (P. acutifolius A. Gray) were compared for the amount of introgression occurring between genomes by amplified fragment length polymorphism (AFLP) markers. A total of 60 genotypes we...
Muñoz Flórez, Ligia Carmenza
,
Blair, Matthew W.
,
Duque E., Myriam Cristina
,
Tohme, Joseph M.
,
Roca, William M.
,
[Introgression in common bean x tepary bean interspecific congruity-backcross line as measured by AFLP markers]
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Introgression in common bean x tepary bean interspecific congruity-backcross line as measured by AFLP markers
Se estima que la población mundial en el año 2020 será de 7.5 billones de habitantes, con más del 80% viviendo en los países en desarrollo (FAO, 1999). Aunque los datos globales sobre producción de alimentos superan el crecimiento de la población, un análisis por regiones o países (FAO, 1993) rev...
Roca, William M.
,
[Biotecnología y seguridad alimentaria en los países en desarrollo]
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Biotecnología y seguridad alimentaria en los países en desarrollo