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Report 1992

Autor: Roca, William M. Henry, Guy Angel, F. Sarria, R

CIAT activities in developing biotechnology applications to cassava in the last decade are described. The strategies to identify research topics and implement in house capabilities and an international cooperative effort in cassava biotechnology are emphasized. Successful integration of selected ...

Enlace original: https://cgspace.cgiar.org/handle/10568/71401

Investigación en Agricultura
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Roca, William M. ,  Henry, Guy ,  Angel, F. ,  Sarria, R ,  [Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)] ,  Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)
Report 1980

Autor: Roca, William M.

Plant tissue culture includes a wide range of techniques: protoplast, cell, anther, callus, meristem and organ culture, all having one common factor: the growth of vegetative material in an aseptic environment; i,e., the sterilized culture medium in test tubes. In spite of the fact that several o...

Enlace original: https://cgspace.cgiar.org/handle/10568/71800

Investigación en Agricultura
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Roca, William M. ,  [Cultivo de tejidos en yuca] ,  Cultivo de tejidos en yuca
Report 1981

Autor: Roca, William M.

Methods of vegetative reproduction are discussed using the results obtained with cassava and potato. Results of clone cleaning by heat therapy and in vitro meristem culture are given. Two methods for rapid cassava multiplication are proposed: by sprouts from cuttings and single leaf cuttings, whi...

Enlace original: https://cgspace.cgiar.org/handle/10568/71822

Investigación en Agricultura
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Roca, William M. ,  [Reproduccion vegetativa de materiales para la siembra libres de enfermedades] ,  Reproduccion vegetativa de materiales para la siembra libres de enfermedades
Journal Article 1987

Autor: Chavez, R. Roca, William M. Williams, J.T.

The overall and specific objectives of a collaborative research project between the International Board for Plant Genetic Resources (IBPGR) and CIAT are given. The overall objective is to assess and demonstrate the technical and logistic aspects of establishing and operating a pilot in vitro acti...

Enlace original: https://cgspace.cgiar.org/handle/10568/88602

Investigación en Agricultura
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Chavez, R. ,  Roca, William M. ,  Williams, J.T. ,  [IBPGR-CIAT Collaborative Project on a pilot in vitro active genebank] ,  IBPGR-CIAT Collaborative Project on a pilot in vitro active genebank
Journal Article 1989

Autor: Roca, William M. Chavez, R. Martin, M.L.. Arias, D.I. Mafla, G. Reyes, Raúl

For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro ...

Enlace original: https://cgspace.cgiar.org/handle/10568/83252

Investigación en Agricultura
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Roca, William M. ,  Chavez, R. ,  Martin, M.L.. ,  Arias, D.I. ,  Mafla, G. ,  Reyes, Raúl ,  [In vitro methods of germ-plasm conservation] ,  In vitro methods of germ-plasm conservation
Manual 1979

Autor: Roca, William M.
Enlace original: https://cgspace.cgiar.org/handle/10568/54163

Investigación en Agricultura
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Roca, William M. ,  [Meristem culture in cassava: principles and methodology: principles and procedures] ,  Meristem culture in cassava: principles and methodology: principles and procedures
Book Chapter 1991

Autor: Roca, William M. Nunez, VM Mornan, K

Different aspects of anther culture are reviewed as follows: its application in plant breeding, conditions that affect results (physiology and genotype of donor plants, albinism, pollen development, pretreatment of anthers, culture media), var. obtained by anther culture, and comparative advantag...

Enlace original: https://cgspace.cgiar.org/handle/10568/55094

Investigación en Agricultura
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Roca, William M. ,  Nunez, VM ,  Mornan, K ,  [Cultivo de anteras y mejoramiento de plantas] ,  Cultivo de anteras y mejoramiento de plantas
Book Chapter 1991

Autor: Ramírez, H. Calderón, A. Roca, William M.

Several important aspects of the use of molecular markers in cassava germplasm characterization and in plant breeding programs are indicated. Basic concepts considered include protein and DNA electrophoresis. In the case of cassava, isoenzyme electrophoresis was applied to confirm duplicates dete...

Enlace original: https://cgspace.cgiar.org/handle/10568/55097

Investigación en Agricultura
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Ramírez, H. ,  Calderón, A. ,  Roca, William M. ,  [Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal] ,  Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal
Book Chapter 1991

Autor: Roca, William M. Nolt, Barry L. Mafla, G. Roa E, J.C. Reyes, R.

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...

Enlace original: https://cgspace.cgiar.org/handle/10568/55095

Investigación en Agricultura
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Roca, William M. ,  Nolt, Barry L. ,  Mafla, G. ,  Roa E, J.C. ,  Reyes, R. ,  [Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)] ,  Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)
Book Chapter 1991

Autor: Roca, William M. Arias, D.I. Chavez, R.

In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2...

Enlace original: https://cgspace.cgiar.org/handle/10568/55096

Investigación en Agricultura
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Roca, William M. ,  Arias, D.I. ,  Chavez, R. ,  [Metodos de conservacion in vitro del germoplasma] ,  Metodos de conservacion in vitro del germoplasma

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