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Ceballos, H.Fregene, Martin A.Roca, William M.Tohme, Joseph M.[Cassava: an ideal crop for biotechnology tools]Cassava: an ideal crop for biotechnology tools

CBN is an informal forum for cassava biotechnologists, collaborating research and development specialists, and cassava research users. CBN`s research goals are genetic and processing improvements for cassava. Its development goal is to encourage interdisciplinary research in national programs of ...


Thro, Ann MarieBonierbale, Merideth W.Roca, William M.Best, R.Henry, Guy[Why, what, who and when: an overview of the Cassava Biotechnology Network (CBN) and current cassava biotechnology research worldwide]Why, what, who and when: an overview of the Cassava Biotechnology Network (CBN) and current cassava biotechnology research worldwide

Cassava is mostly growing by resource-limited farmers in the Americas, Africa, Asia and Oceania. The crop is an important source of food and income for millions in the tropics. Vegetative propagation, bulkiness of planting material and risk of genetic erosion make cassava an ideal target for inno...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[Cryopreservation for long-term conservation of cassava genetic resources]Cryopreservation for long-term conservation of cassava genetic resources

To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains th...


Sarria, RABalcázar, NDestéfano Beltrán, L.J.C.Roca, William M.[Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)]Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...


Gómez, R.Angel, F.Bonierbale, Merideth W.Rodríguez, F.Tohme, Joseph M.Roca, William M.[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]Selecting heterozygous parents and single-dose markers for genetic mapping in cassava
Henshaw, GGRoca, William M.[Report of the working group on Manihot germplasm conservation, exchange and micropropagation]Report of the working group on Manihot germplasm conservation, exchange and micropropagation

Our strategy for Aqrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Aqrobacterium strains as well as regeneration protocols have been developed; the plasmid ...


Sarria, RAGómez, A.Cataño C, MLHerrera, P.V.Calderón, A.Mayer, Jorge EdgardRoca, William M.[Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation]Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation

Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pol...


Cataño C, MLMornan, KPlazas, JRoca, William M.[Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos]Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos
Mafla, G.Roca, William M.Reyes, R.Roa E, J.C.Muñoz M, L.Baca, AEIwanaga, Masaru[In vitro management of cassava germplasm at CIAT]In vitro management of cassava germplasm at CIAT

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