Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...
Enlace original:
https://cgspace.cgiar.org/handle/10568/83250
Escobar Pérez, Roosevelt H.
,
Mafla, G.
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Roca, William M.
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[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]
,
A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen