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Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vit...


González Amao, M.T.Panta, A.Roca, William M.Escobar Pérez, Roosevelt H.Engelmann, F.[Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops]Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro ...


Roca, William M.Chavez, R.Martin, M.L..Arias, D.I.Mafla, G.Reyes, Raúl[In vitro methods of germ-plasm conservation]In vitro methods of germ-plasm conservation

Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were ...


Marin, M.L.Mafla, G.Roca, William M.Withers, Lyndsey A.[Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen]Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen

Cassava (Manihot esculenta Crantz) is a tough and productive survival crop of smallholder farmers in the tropics. It is often grown where other crops fail. Research can make important contribution to food security and economic development in cassava-growing areas. This can be enhanced through in-...


Thro, Ann MarieRoca, William M.Iglesias, C.A.Henry, GuyNg, S.Y.C.[Contributions of in-vitro biology to cassava improvement]Contributions of in-vitro biology to cassava improvement

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