A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...
Fregene, Martin A.
,
Angel, F.
,
Gómez, R.
,
Rodríguez, F.
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Tohme, Joseph M.
,
Bonierbale, Merideth W.
,
[A molecular genetic map of cassava (Manihot esculenta Crantz)]
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A molecular genetic map of cassava (Manihot esculenta Crantz)
CIAT activities in developing biotechnology applications to cassava in the last decade are described. The strategies to identify research topics and implement in house capabilities and an international cooperative effort in cassava biotechnology are emphasized. Successful integration of selected ...
Roca, William M.
,
Henry, Guy
,
Angel, F.
,
Sarria, R
,
[Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)]
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Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)
Fregene, Martin A.
,
Tohme, Joseph M.
,
Roca, William M.
,
Chavarriaga Aguirre, Paul
,
Escobar Pérez, Roosevelt H.
,
Ceballos, H.
,
[Biotecnología para la yuca]
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Biotecnología para la yuca
Ramírez, H.
,
Calderón, A.
,
Roca, William M.
,
[Técnicas moleculares para evaluar y mejorar el germoplasma vegetal]
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Técnicas moleculares para evaluar y mejorar el germoplasma vegetal
Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...
Fregene, Martin A.
,
Okogbenin, Emmanuel
,
Mba, C
,
Angel, F.
,
Suarez, MC
,
Gutiérrez A., Janeth Patricia
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Bonierbale, Merideth W.
,
Tohme, Joseph M.
,
[Genome mapping in cassava improvement: Challenges, achievements and opportunities]
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Genome mapping in cassava improvement: Challenges, achievements and opportunities
Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...
Gómez, R.
,
Angel, F.
,
Bonierbale, Merideth W.
,
Rodríguez, F.
,
Tohme, Joseph M.
,
Roca, William M.
,
[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]
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Selecting heterozygous parents and single-dose markers for genetic mapping in cassava
The construction of a detailed genetic map of cassava (Manihot esculenta Crantz) will be useful to tag agronomically important trais as well as to isolate and to clone cassava genes in the future. This map will also contribute to the understanding of cassava genomic structure and evolution. As a ...
Angel, F.
,
Giraldo, F.
,
Gómez, R.
,
Iglesias, C.A.
,
Tohme, Joseph M.
,
Roca, William M.
,
[Use of RFLPs and RAPDs in cassava genomic studies]
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Use of RFLPs and RAPDs in cassava genomic studies
Several important aspects of the use of molecular markers in cassava germplasm characterization and in plant breeding programs are indicated. Basic concepts considered include protein and DNA electrophoresis. In the case of cassava, isoenzyme electrophoresis was applied to confirm duplicates dete...
Ramírez, H.
,
Calderón, A.
,
Roca, William M.
,
[Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal]
,
Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal
Angel, F.
,
Arias Garzón, D.I.
,
Roca, William M.
,
[DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR]
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DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR