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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)
Thro, Ann MarieBeachy, Roger N.Bonierbale, Merideth W.Fauquet, Claude M.Henry, GuyHenshaw, Graham G.Hughes, Monica A.Kawano, KazuoRaemakers, C.J.J.M.Roca, William M.Sch"pke, C.Taylor, Nigel J.Visser, Richard G.F.[International research on biotechnology of cassava (tapioca, Manihot esculenta Crantz) and its relevance to Southeast Asian economies: a cassava biotechnology network review]International research on biotechnology of cassava (tapioca, Manihot esculenta Crantz) and its relevance to Southeast Asian economies: a cassava biotechnology network review

The cassava core collection (601 genotypes) was evaluated for root and leaf contents of micronutrient minerals, ascorbic acid, and carotene. Wide genetic variability was observed for all measurements, indicating that there is good potential for exploiting and improving the nutritive value of cass...


Chavez, Alba L.Bedoya Flores, JMSánchez, T.Iglesias, C.A.Ceballos, H.Roca, William M.[Iron, carotene, and ascorbic acid in cassava roots and leaves]Iron, carotene, and ascorbic acid in cassava roots and leaves

Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...


Angel, F.Barney, Victoria E.Tohme, Joseph M.Roca, William M.[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a b...


Chavarriaga Aguirre, PaulBrand, AlejandroMedina, AdrianaPrías, MónicaEscobar Pérez, Roosevelt H.Martínez, JuanDíaz, PaulaLópez Carrascal, Camilo ErnestoRoca, William M.Tohme, Joseph M.[The potential of using biotechnology to improve cassava: a review]The potential of using biotechnology to improve cassava: a review

The overall and specific objectives of a collaborative research project between the International Board for Plant Genetic Resources (IBPGR) and CIAT are given. The overall objective is to assess and demonstrate the technical and logistic aspects of establishing and operating a pilot in vitro acti...


Chavez, R.Roca, William M.Williams, J.T.[IBPGR-CIAT Collaborative Project on a pilot in vitro active genebank]IBPGR-CIAT Collaborative Project on a pilot in vitro active genebank

For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro ...


Roca, William M.Chavez, R.Martin, M.L..Arias, D.I.Mafla, G.Reyes, Raúl[In vitro methods of germ-plasm conservation]In vitro methods of germ-plasm conservation

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen

Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were ...


Marin, M.L.Mafla, G.Roca, William M.Withers, Lyndsey A.[Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen]Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen
Fregene, Martin A.Ospina, Julio A.Roca, William M.[Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos]Recovery of cassava (Manihot esculenta Crantz) plants from culture of immature zygotic embryos

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