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Reports the work in progress at ILCA on appropriate methods of collecting Digitaria species and multiplying these former species and Leucaena leucocephala, Erythrina brucei and Sesbania sesban in vitro. Discusses their potential use in forage genetic resources. Discusses inoculation of stem cutti...


Ruredzo, T.J.Hanson, Jean[Practical applications of in-vitro techniques to forage germplasm]Practical applications of in-vitro techniques to forage germplasm

In vitro cultures were initiated from shoots taken from seedlings of Faidherbia albida on Murashige and skoog based medium supplemented with different combinations of 6-benzylaminopurine (BA) and alpha-raphthaleneacetic acid (NAA). The shoots grew and rooted on all media. Rooting and vigorous gro...


Ruredzo, T.J.Hanson, Jean[Plant recovery from seedling-derived shoot tips of Faidherbia albida grown in vitro]Plant recovery from seedling-derived shoot tips of Faidherbia albida grown in vitro

Conservation of germplasm has traditionally relied to a large extent on storage of seeds in genebanks. However, some forage species may take a long time to research maturity and produce seeds or, in the case of grasses, have inadequate production of seeds for collection, multiplication, dissemina...


Hanson, JeanMtambo, P.J.Ruredzo, T.J.[Conservation of forage germplasm with an emphasis on application of tissue culture techniques in a genebank]Conservation of forage germplasm with an emphasis on application of tissue culture techniques in a genebank

Hanson, JeanMtambo, P.J.Ruredzo, T.J.[Conservation of forage germplasm with an emphasis on application of tissue culture techniques in a genebank]Conservation of forage germplasm with an emphasis on application of tissue culture techniques in a genebank
Autor: Ruredzo, T.J.

Simple in vitro techniques were developed for collecting Digitaria eriantha ssp. pentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface- sterilis...


Ruredzo, T.J.[A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon]A minimum facility method for in vitro collection of Digitaria eriantha ssp. pentzii and Cynodon dactylon

Discusses tissue culture techniques used on some accessions of Cynodon and Digitaria at the Zwai seed multiplication site of ILCA for maintainance and distribution of germplasm. Presents data on the establishment survival and re-establishment of cultures.


Ruredzo, T.J.Hanson, Jean[Tissue culture techniques for maintenance and distribution of germplasm of tropical grasses]Tissue culture techniques for maintenance and distribution of germplasm of tropical grasses

Looks into ILCA'S genebank which has a collection of over 9000 accessions of forage germplasm of grasses, legumes and browse. The majority of accessions are stored by conventional methods of seed storage confirming to international standards as far as possible. However, there are some species of ...


Hanson, JeanRuredzo, T.J.[Germplasm storage at ILCA]Germplasm storage at ILCA

Briefly outlines results of a research carried out at ILCA since 1986 on in vitro culture technology of Cynodon aethiopicus, C. dactylon, Digitaria decumbens, Sesbania sesban, Leucaena leucocephala, Erythrina brucei, Faidherbia albida and Acacia tortilis.


Hanson, JeanRuredzo, T.J.[In vitro culture techniques for forage genetic resources]In vitro culture techniques for forage genetic resources
Autor: Ruredzo, T.J.

Simple in vitro techniques were developed for collecting Digitaria eriantha sspentzii and Cynodon dactylon to obviate deterioration, quarantine and bulk problems of conventional methods for collecting vegetative material. Various antimicrobial agents were tested for effective surface-sterilizatio...


Ruredzo, T.J.[A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon]A minimum facility method for in vitro collection of Digitaria eriantha sspentzii and Cynodon dactylon

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