Bananas are a staple food in Eastern Africa, with 25.3% of the total world production. The production is, however, threatened by the presence of several diseases, of which the fungal diseases black sigatoka and Panama disease are the most important. With the development of embryogenic cell suspen...
Smet, K. de
,
Panis, B.
,
Sagi, L.
,
Cammue, B.
,
Swennen, R.
,
[Improvement of bananas for black sigatoka and Panama disease resistance through genetic manipulation]
,
Improvement of bananas for black sigatoka and Panama disease resistance through genetic manipulation
Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt select...
Kovacs, G.
,
Sagi, L.
,
Jacon, G.
,
Arinaitwe, G.
,
Busogoro, J.P.
,
Thiry, E.
,
Strosse, H.
,
Swennen, R.L.
,
Remy, S.
,
[Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease]
,
Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease
Swennen, R.L.
,
Carpentier, S.C.
,
Henry, I.M.
,
Vertommen, A.
,
Houwe, I. van den
,
Kovacs, G.
,
Sagi, L.
,
Remy, S.
,
Panis, B.
,
[From fundamental research discoveries to applications for banana improvement]
,
From fundamental research discoveries to applications for banana improvement
In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeri...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Panis, B.
,
Cammue, B.P.
,
Volckaert, G.
,
Swennen, R.
,
[Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions]
,
Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions
The term banana is meant throughout this chapter to cover dessert and cooking bananas as well as plantains. With an estimated total production of more than 76 million tons in 1992 (FAO 1993), banana is the no. 1 fruit crop in the world. Ninety-nine percent of world's annual production is cultivat...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Swennen, R.
,
Panis, B.
,
[Genetic transformation in Musa species (Banana)]
,
Genetic transformation in Musa species (Banana)
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...
Bananas are monocots and members of the genus Musa, family Musaceae. They are the second largest fruit crop in the world with an annual production of about 65 million tons (INIBAP 1993). The export trade, involving just dessert bananas of the Cavendish subgroup, comprises only 10% of the total ba...
Panis, B.
,
Sagi, L.
,
Swennen, R.
,
[Regeneration of plants from protoplasts of Musa species (banana)]
,
Regeneration of plants from protoplasts of Musa species (banana)