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Journal Article 2000

Autor: Sarria, RA Torres Castro, Edgar Alonso Angel, F. Chavarriaga Aguirre, Paul Roca, William M.

Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...

Enlace original: https://cgspace.cgiar.org/handle/10568/44199

Investigación en Agricultura
Enlace permanente
Sarria, RA ,  Torres Castro, Edgar Alonso ,  Angel, F. ,  Chavarriaga Aguirre, Paul ,  Roca, William M. ,  [Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation] ,  Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation
Conference Paper 1995

Autor: Sarria, RA Balcázar, N Destéfano Beltrán, L.J.C. Roca, William M.

To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains th...

Enlace original: https://cgspace.cgiar.org/handle/10568/55850

Investigación en Agricultura
Enlace permanente
Sarria, RA ,  Balcázar, N ,  Destéfano Beltrán, L.J.C. ,  Roca, William M. ,  [Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)] ,  Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)
Conference Paper 1993

Autor: Sarria, RA Gómez, A. Cataño C, ML Herrera, P.V. Calderón, A. Mayer, Jorge Edgard Roca, William M.

Our strategy for Aqrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Aqrobacterium strains as well as regeneration protocols have been developed; the plasmid ...

Enlace original: https://cgspace.cgiar.org/handle/10568/55690

Investigación en Agricultura
Enlace permanente
Sarria, RA ,  Gómez, A. ,  Cataño C, ML ,  Herrera, P.V. ,  Calderón, A. ,  Mayer, Jorge Edgard ,  Roca, William M. ,  [Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation] ,  Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation
Conference Paper 1993

Autor: Sarria, RA Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, William M.

Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were...

Enlace original: https://cgspace.cgiar.org/handle/10568/55686

Investigación en Agricultura
Enlace permanente
Sarria, RA ,  Ocampo Nahar, César H. ,  Ramírez, H. ,  Hershey, Clair H. ,  Roca, William M. ,  [Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz)] ,  Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz)

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