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To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains th...


Sarria, RABalcázar, NDestéfano Beltrán, L.J.C.Roca, William M.[Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)]Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)

Our strategy for Aqrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Aqrobacterium strains as well as regeneration protocols have been developed; the plasmid ...


Sarria, RAGómez, A.Cataño C, MLHerrera, P.V.Calderón, A.Mayer, Jorge EdgardRoca, William M.[Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation]Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation

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