The effects of 0.2% aqueous solution of colchicine on the survival rate and morphological features of five accessions of Dioscorea alata were investigated. Sprouting buds of two month old plants were treated with 0.2% colchicine and their performances were monitored until maturity. Survival of bu...
Ajayi, A.T.
,
Adesoye, A.I.
,
Asiedu, Robert
,
Sartie, A.M.
,
[Colchicine-induced variations in survival rate and morphological characteristics of water yam (Dioscorea alata)]
,
Colchicine-induced variations in survival rate and morphological characteristics of water yam (Dioscorea alata)
Progress is being made at the International Institute for Tropical Agriculture to develop molecular tools for marker-assisted selection that would complement and expedite conventional breeding approaches for genetic improvement of yams (Dioscorea spp.). F1 mapp...
Sartie, A.M.
,
Asiedu, Robert
,
[Development of mapping populations for genetic analysis in yams (Dioscorea rotundata and D. alata)]
,
Development of mapping populations for genetic analysis in yams (Dioscorea rotundata and D. alata)
Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...
Sharma, K.
,
Bhattacharjee, R.
,
Sartie, A.M.
,
Kumar, P.L.
,
[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]
,
An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
Background: Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence inform...
Narina, S.S.
,
Buyyarapu, R.
,
Kottapalli, K.R.
,
Sartie, A.M.
,
Ali, M.I.
,
Asiedu, Robert
,
Hodeba, M.
,
Sayre, B.L.
,
Scheffler, B.E.
,
[Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)]
,
Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)
Yam (
Dioscorea
spp.) is a major staple crop
widely cultivated for its starchy tubers. To date,
very few marker resources are publicly avail
-
able as tools for genetic and genomic studies of
this economically important crop. In this study,
90 simple sequence repeat (SSR) markers were
devel...
Tamiru, M.
,
Yamanaka, S.
,
Mitsuoka, C.
,
Babil, P.
,
Takagi, H.
,
López Montes, A.
,
Sartie, A.M.
,
Asiedu, Robert
,
Terauchi, R.
,
[Development of genomic simple sequence repeat markers for yam]
,
Development of genomic simple sequence repeat markers for yam
To determine whether or not FT-IR spectroscopy could be used for taxonomic and metabolic discriminationof African yam lines, tuber samples from African and Asian yam species were subjected to FT-IR.Most remarkable spectral differences between African and Asian yams were found in the 1750–1700 cm ...
A working collection of yam (Dioscoreaspp.) comprising 53 landraces and seven improved cultivars of four species (D. alata L., D. cayenensis Lam., D. dumetorum (Kunth) and D. rotundata Poir.) was evaluated for phenotypic and genetic diversity. The evaluation involved field assessment of 24 morpho...
Sartie, A.M.
,
Asiedu, Robert
,
Franco, J.
,
[Genetic and phenotypic diversity in a germplasm working collection of cultivated tropical yams (Dioscorea spp.)]
,
Genetic and phenotypic diversity in a germplasm working collection of cultivated tropical yams (Dioscorea spp.)
Conventional breeding takes several years to achieve homozygosity in yam. Anther culture technology is a rapid and viable alternative for the development of homozygous plants, therefore optimizing key parameters for the establishment of an efficient protocol for yam anther culture is indispensabl...