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Svitek, NicholasSaya, R.Awino, EliasGilbert, S.C.Poole, Elizabeth J.Nene, VishvanathSteinaa, Lucilla[Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination]Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination
Svitek, NicholasSaya, R.Awino, EliasMunyao, S.Njoroge, T.Gilbert, S.Nene, VishvanathSteinaa, Lucilla[Evaluating the Ad5/MVA antigen delivery systems]Evaluating the Ad5/MVA antigen delivery systems

Awino, EliasAye, R.Bertrand, S.Gesharisha, J.Gicheru, N.Jores, JoergLiljander, Anne M.Muriuki, C.Naessens, JanNene, VishvanathNjoroge, T.Nyongesa, S.Pelle, RogerSsajjakambwe, P.Saya, R.Schieck, Elise G.Steinaa, LucillaSvitek, NicholasWasilwa, J.[ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)]ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)
Sileghem, M.R.Saya, R.Ellis, J.Flynn, J.N.Peel, J.E.Williams, D.J.L.[Detection and neutralization of bovine tumor necrosis factor by a monoclonal antibody]Detection and neutralization of bovine tumor necrosis factor by a monoclonal antibody

Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradien...


Svitek, NicholasTaracha, E.L.N.Saya, R.Awino, EliasNene, VishvanathSteinaa, Lucilla[Analysis of the cellular immune responses to vaccines]Analysis of the cellular immune responses to vaccines

East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ...


Akoolo, L.Pelle, RogerSaya, R.Awino, EliasNyanjui, J.Taracha, E.L.N.Kanyari, P.Mwangi, D.M.Graham, S.P.[Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle]Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle

The mechanisms whereby trypanotolerant N'Dama cattle control infection with Trypanosoma congolense are unknown. Previous studies have suggested that the monocytes of N'Dama cattle are more highly activated during infection than those of trypanosus ceptible Boan cattle. However, we have recently r...


Taylor, K.Mertens, B.Lutje, V.Saya, R.[Trypanosoma congolense infection of trypanotolerant N'Dama (Bos taurus) cattle is associated with decreased secretion of nitric oxide by interferon-gamma-activated monocytes and increased transcription of interleukin-10]Trypanosoma congolense infection of trypanotolerant N'Dama (Bos taurus) cattle is associated with decreased secretion of nitric oxide by interferon-gamma-activated monocytes and increased transcription of interleukin-10

The membrane-associated form of the variable surface glycoprotein (mfVSG) from African trypanosomes is a potent macrophage activator capable of inducing production of tumor necrosis factor z (TNFalfa) in both bovine and murine models. Using a bovine model. we have re-investigated the hypothesis t...


Sileghem, M.R.Saya, R.Grab, D.J.Naessens, Jan[An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes]An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes

Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...


Graham, S.P.Saya, R.Awino, EliasNgugi, D.Nyanjui, J.K.Hecker, R.Taracha, E.L.N.Nene, Vishvanath[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva
Sileghem, M.R.Flynn, J.N.Saya, R.Williams, D.J.L.[Secretion of co-stimulatory cytokines by monocytes and macrophages during infection with Trypanosoma (Nannomonas) congolense in susceptible and tolerant cattle]Secretion of co-stimulatory cytokines by monocytes and macrophages during infection with Trypanosoma (Nannomonas) congolense in susceptible and tolerant cattle

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