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Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...


Wambugu, J.Kanguha, E.Hanotte, Olivier H.Davis, S.Taylor, J.Skow, L.Brunner, R.Schwerin, M.Teale, A.J.Iraqi, F.A.[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone

The paper first describes interleukin-12 P35 encoding gene and discusses its role. It then looks into PCR conditions and sequencing; genotyping; sequencing; polymorphism frequency; mendelian inheritance; chromosomal location in sheep and cattle of different species.


Schmidt, P.Kuhn, C.Kang'a, S.Hanotte, Olivier H.Vanselow, J.Anton, I.Langner, ChSchwerin, M.[Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4]Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4

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