A preliminary search for QTLs in a cattle F2 resource populations segregating for trypanotolerance has revealed three large candidate chromosomal regions (Bta02, Bta05, Bta07). In order to narrow down the position of the trypanotolerant QTLs, we are building comparative chromosomal maps between c...
Nilsson, P.H.
,
Kang'a, S.
,
Rottengatter, K.
,
Suedbeck, U.
,
Iraqui, F.
,
Mwakaya, J.
,
Mwangi, D.
,
Womack, J.E.
,
Goldammer, T.
,
Schwerin, M.
,
Bradley, D.
,
Agaba, Morris
,
Sugimoto, K.
,
Gelhaus, A.
,
Horstmann, R.
,
Teale, A.J.
,
Kemp, Stephen J.
,
Hanotte, Olivier H.
,
[Radiation hybrid maps of candidate trypanotolerance regions in cattle]
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Radiation hybrid maps of candidate trypanotolerance regions in cattle
An alternative approach for the direct analysis of chromosome regions corresponding to economical traits, based on chromosome microdissection, is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by fluorescence in ...
Goldammer, T.
,
Brunner, R.M.
,
Kang'a, S.
,
Hanotte, Olivier H.
,
Schwerin, M.
,
[Establishment of bovine chromosome region specific high resolution maps using microdissection]
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Establishment of bovine chromosome region specific high resolution maps using microdissection
Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...
Wambugu, J.
,
Kanguha, E.
,
Hanotte, Olivier H.
,
Davis, S.
,
Taylor, J.
,
Skow, L.
,
Brunner, R.
,
Schwerin, M.
,
Teale, A.J.
,
Iraqi, F.A.
,
[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]
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Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
The paper first describes interleukin-12 P35 encoding gene and discusses its role. It then looks into PCR conditions and sequencing; genotyping; sequencing; polymorphism frequency; mendelian inheritance; chromosomal location in sheep and cattle of different species.
Schmidt, P.
,
Kuhn, C.
,
Kang'a, S.
,
Hanotte, Olivier H.
,
Vanselow, J.
,
Anton, I.
,
Langner, Ch
,
Schwerin, M.
,
[Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4]
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Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4
A two species comparative genome scanning approach has been taken to mapping, and eventually identifying, genes at quantitative trait loci (QTL) influencing resistance to trypanosomosis due to infection with Trypanosoma congolense. Using two large murine F2 and two F6 advanced intercross populati...
Teale, A.J.
,
Agaba, Morris
,
Clapcott, S.J.
,
Gelhaus, A.
,
Haley, C.
,
Psifidis, A.
,
Horstmann, R.
,
Iraqui, F.
,
Kemp, Stephen J.
,
Nilsson, P.
,
Schwerin, M.
,
Sekikawa, K.
,
Soller, M.
,
Sugimoto, Y.
,
Womack, J.
,
[Resistance to trypanosomosis: Of markers, genes and mechanisms]
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Resistance to trypanosomosis: Of markers, genes and mechanisms
Trypanosomiasis is one of the most important diseases in African cattle. About 3 million cattle die every year by this disease. However, about 5 percent of the total African cattle have a natural resistance against trypanosomiasis. In a preliminary study markers linked to trypanotolerance have be...
Goldammer, T.
,
Brunner, R.M.
,
Kang'a, S.
,
Hanotte, Olivier H.
,
Schwerin, M.
,
[Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance]
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Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance