Conditions were established to obtain depletion of T lymphocyte subsets in lymphoid tissues of calves by injection of mouse monoclonal antibodies to T cell antigens. Adverse reactions were avoided by injecting small quantities of antibody until target cells had disappeared from blood. Two differe...
Naessens, Jan
,
Scheerlinck, J.P.
,
Buysscher, E.V. de
,
Kennedy, D.
,
Sileghem, M.R.
,
[Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies]
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Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies
Natural resistance to African trypanosomiasis in certain Bos taurus cattle in West Africa, called trypanotolerance, may hold solutions for control of this economically crippling disease. Comparison of immune responses between trypanotolerant and trypanosusceptible cattle have shown some differen...
Naessens, Jan
,
Teale, A.J.
,
Sileghem, M.R.
,
[Identification of mechanisms of natural resistance to African trypanosomiasis in cattle]
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Identification of mechanisms of natural resistance to African trypanosomiasis in cattle
Naessens, Jan
,
Sileghem, M.R.
,
MacHugh, Niall D.
,
Park, Y.H.
,
Davis, W.C.
,
Toye, Philip G.
,
[Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene]
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Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
Naessens, Jan
,
Grab, D.J.
,
Sileghem, M.R.
,
[Identifying the mechanisms of trypanotolerance in cattle]
,
Identifying the mechanisms of trypanotolerance in cattle
The membrane-associated form of the variable surface glycoprotein (mfVSG) from African trypanosomes is a potent macrophage activator capable of inducing production of tumor necrosis factor z (TNFalfa) in both bovine and murine models. Using a bovine model. we have re-investigated the hypothesis t...
Sileghem, M.R.
,
Saya, R.
,
Grab, D.J.
,
Naessens, Jan
,
[An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes]
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An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes
Sileghem, M.R.
,
Flynn, J.N.
,
[Suppression of interleukin 2 secretion and interleukin 2 receptor expression during tsetse-transmitted trypanosomiasis in cattle]
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Suppression of interleukin 2 secretion and interleukin 2 receptor expression during tsetse-transmitted trypanosomiasis in cattle
Mice infected with African trypanosomes produce exceptionally large amounts of serum IgM, a major part of which binds to non-trypanosome antigens such as trinitrophenol and single-strand DNA. In this paper, we describe that in cattle infected with Trypanosoma congolense and T. vivax, similar anti...
Buza, J.J.
,
Sileghem, M.R.
,
Gwakisa, P.S.
,
Naessens, Jan
,
[CD5+B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense infected cattle]
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CD5+B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense infected cattle
Flynn, J.N.
,
McKeever, Declan J.
,
Sileghem, M.R.
,
Naessens, Jan
,
[Modulation of the phenotype and function of bovine afferent lymph cells during infection with Trypanosoma congolense]
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Modulation of the phenotype and function of bovine afferent lymph cells during infection with Trypanosoma congolense
Sileghem, M.R.
,
Flynn, J.N.
,
Logan-Henfrey, L.L.
,
Ellis, J.
,
[Tumour necrosis factor production by monocytes from cattle infected with Trypanosoma (Duttonella) vivax and Trypanosoma (Nannomonas) congolense STI possible association with severity of anaemia associated with the disease]
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Tumour necrosis factor production by monocytes from cattle infected with Trypanosoma (Duttonella) vivax and Trypanosoma (Nannomonas) congolense STI possible association with severity of anaemia associated with the disease
Flynn, J.N.
,
Sileghem, M.R.
,
Williams, D.J.L.
,
[Parasite-specific T-Cell response of trypanotolerant and trypanosusceptible cattle during infection with Trypanosoma congolense]
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Parasite-specific T-Cell response of trypanotolerant and trypanosusceptible cattle during infection with Trypanosoma congolense