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Conditions were established to obtain depletion of T lymphocyte subsets in lymphoid tissues of calves by injection of mouse monoclonal antibodies to T cell antigens. Adverse reactions were avoided by injecting small quantities of antibody until target cells had disappeared from blood. Two differe...


Naessens, JanScheerlinck, J.P.Buysscher, E.V. deKennedy, D.Sileghem, M.R.[Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies]Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies

Natural resistance to African trypanosomiasis in certain Bos taurus cattle in West Africa, called trypanotolerance, may hold solutions for control of this economically crippling disease. Comparison of immune responses between trypanotolerant and trypanosusceptible cattle have shown some differen...


Naessens, JanTeale, A.J.Sileghem, M.R.[Identification of mechanisms of natural resistance to African trypanosomiasis in cattle]Identification of mechanisms of natural resistance to African trypanosomiasis in cattle
Naessens, JanSileghem, M.R.MacHugh, Niall D.Park, Y.H.Davis, W.C.Toye, Philip G.[Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene]Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
Ellis, J.A.Fish, W.R.Sileghem, M.R.McOdimba, F.A.[A calorimetric assay for trypanosome viability and metabolic function]A calorimetric assay for trypanosome viability and metabolic function
Sileghem, M.R.Saya, R.Ellis, J.Flynn, J.N.Peel, J.E.Williams, D.J.L.[Detection and neutralization of bovine tumor necrosis factor by a monoclonal antibody]Detection and neutralization of bovine tumor necrosis factor by a monoclonal antibody

Murine models have suggested that CD8 T cells might play a major parasite-promoting role in African trypanosomiasis. To assess the role of these cells in a natural host environment, we have depleted CD8 cells from Boran cattle in vivo and subsequently infected these animals with Trypanosoma congo...


Sileghem, M.R.Naessens, Jan[Are CD8 T cells involved in control of African trypanosomiasis in a natural host environment?]Are CD8 T cells involved in control of African trypanosomiasis in a natural host environment?

Natural resistance to African trypanosomiasis in certain Bos taurus cattle in West Africa, called trypanotolerance, may hold solutions for control of this economically crippling disease. Comparison of immune responses between trypanotolerant and trypanosusceptible cattle have shown some differenc...


Naessens, JanTeale, A.J.Sileghem, M.R.[Identification of mechanisms of natural resistance to African trypanosomiasis in cattle]Identification of mechanisms of natural resistance to African trypanosomiasis in cattle

The membrane-associated form of the variable surface glycoprotein (mfVSG) from African trypanosomes is a potent macrophage activator capable of inducing production of tumor necrosis factor z (TNFalfa) in both bovine and murine models. Using a bovine model. we have re-investigated the hypothesis t...


Sileghem, M.R.Saya, R.Grab, D.J.Naessens, Jan[An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes]An accessory role for the diacylglycerol moiety of variable surface glycoprotein of African trypanosomes in the simulation of bovine monocytes

Bovine bone marrow-derived macrophages were infected in vitro with non-cytopathic or cytopathic strains of bovine viral diarrhoea virus. Infection with both biotypes resulted in a decreased production of tumour necrosis factor alpha upon stimulation with heat-inactivated Salmonella dublin or lipo...


Adler, H.Jungi, T.W.Pfister, H.Strasser, M.Sileghem, M.R.Peterhans, E.[Cytokine regulation by virus infection: Bovine viral diarrhea virus, a flavivirus, downregulates production of tumor necrosis factor alpha in macrophages in vitro]Cytokine regulation by virus infection: Bovine viral diarrhea virus, a flavivirus, downregulates production of tumor necrosis factor alpha in macrophages in vitro
Sileghem, M.R.Flynn, J.N.[Suppression of interleukin 2 secretion and interleukin 2 receptor expression during tsetse-transmitted trypanosomiasis in cattle]Suppression of interleukin 2 secretion and interleukin 2 receptor expression during tsetse-transmitted trypanosomiasis in cattle

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