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To date, several viruses of different genera have been reported to infect yam (Dioscorea spp.). The full diversity of viruses infecting yam, however, remains to be explored. High-throughput sequencing (HTS) methods are increasingly being used in the discovery of new plant viral genomes. In this s...


Silva, G.Bömer, M.Rathnayake, A.I.Sewe, S.O.Visendi, P.Oyekanmi, J.O.Quain, M.D.Akomeah, B.Kumar, P.L.Seal, S.E.[Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing]Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing

In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this st...


Bömer, M.Rathnayake, A.I.Visendi, P.Sewe, S.O.Sicat, J.P.A.Silva, G.Kumar, P.L.Seal, S.E.[Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses]Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses

Badnaviruses (family Caulimoviridae, genus Badnavirus) have emerged as serious pathogens especially affecting the cultivation of tropical crops. Badnavirus sequences can be integrated in host genomes, complicating the detection of episomal infections and the assessment of viral genetic diversity ...


Turaki, A.A.Bömer, M.Silva, G.Kumar, P.L.Seal, S.E.[PCR-DGGE analysis: unravelling complex mixtures of badnavirus sequences present in yam germplasm]PCR-DGGE analysis: unravelling complex mixtures of badnavirus sequences present in yam germplasm

Yam mosaic virus (YMV; genus Potyvirus ) is considered to cause the most economically important viral disease of yams ( Dioscorea spp.) in West Africa which is the dominant region for yam production globally. Yams are a vegetatively propagated crop and the use of virus-free planting material form...


Silva, G.Bömer, M.Nkere, C.Kumar, P.L.Seal, S.E.[Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification]Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification

Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particu...


Silva, G.Oyekanmi, J.Nkere, C.K.Bömer, M.Kumar, P.L.Seal, S.E.[Rapid detection of potyviruses from crude plant extracts]Rapid detection of potyviruses from crude plant extracts

Yam (Dioscorea spp.) plants are potentially hosts to a diverse range of badnavirus species (genus Badnavirus, family Caulimoviridae), but their detection is complicated by the existence of integrated badnavirus sequences in some yam genomes. To date, only two badnavirus genomes have been characte...


Bömer, M.Turaki, A.A.Silva, G.Kumar, P.L.Seal, S.E.[A sequence-independent strategy for amplification and characterisation of episomal badnavirus sequences reveals three previously uncharacterised yam badnaviruses]A sequence-independent strategy for amplification and characterisation of episomal badnavirus sequences reveals three previously uncharacterised yam badnaviruses

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