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We determined if laterality of ovulation and intrauterine embryo location differentially induces changes in the mesometrial/endometrial vascularization area (MEVA) between uterine horns, during and after embryo migration, elongation and implantation in llamas. Adult, non-pregnant and non-lactatin...


Ratto, Marcelo H.Urra, FelipeSilva Jiménez, Mauricio[Laterality of Ovulation and Presence of the Embryo Do Not Affect Uterine Horn Blood Flow During the First Month of Gestation in Llamas]Laterality of Ovulation and Presence of the Embryo Do Not Affect Uterine Horn Blood Flow During the First Month of Gestation in Llamas

The aim of this study was to evaluate the effect of increasing incubation temperature of Araucana hen eggs on embryo mortality, hatchability, chick, yolk sac and internal organs weight. Eggs (n=426) were randomly distributed into: a) Control group (n=215), which were incubated at 37.5 degrees C f...


Ruiz Diaz, NancyOrrego, GuillermoReyes, MiguelSilva Jiménez, Mauricio[Increasing Incubation Temperature of Araucana Hen Eggs (Gallus inauris): Effect on Embryo Mortality, Hatchability, Chick, Yolk Sac and Internal Organs Weight]Increasing Incubation Temperature of Araucana Hen Eggs (Gallus inauris): Effect on Embryo Mortality, Hatchability, Chick, Yolk Sac and Internal Organs Weight

The aim of this study was to evaluated changes in endometrial vascularization area (EVA) between the left and right uterine horn: a) during the ovarian follicular growth in intact llamas, and b) after exogenous estradiol administration of estradiol benzoate in ovariectomized (OVX) llamas. In expe...


Silva Jiménez, MauricioUrra, FelipeRatto, Marcelo[Uterine endometrial vascularization during ovarian follicular growth in llamas: The effect of estradiol plasma concentration]Uterine endometrial vascularization during ovarian follicular growth in llamas: The effect of estradiol plasma concentration

Cryopreservation of stallion semen has not reached the level of efficiency and positive results described in other species. This is mainly due to the greater sensitivity of stallion sperm to the freezing process, showing higher rates of oxidative stress and plasma membrane damage, which trigger t...


Jose Contreras, MariaTreulen, FavianElena Arias, MariaSilva Jiménez, MauricioFuentes, FernandaCabrera, PaulinaFelmer, Ricardo[Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology]Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Contents Conventional in vitro fertilization has not yet been implemented in the equine species. One of the main reasons has been the inability to develop a culture medium and incubation conditions supporting high levels of stallion sperm capacitation and hyperactivation in vitro. Although differ...


Arroyo Salvo, CamilaSanhueza, FranciscoFuentes, FernandaTreulen, FavianArias, Maria ElenaCabrera, PaulinaSilva Jiménez, MauricioFelmer, Ricardo[Effect of human tubal fluid medium and hyperactivation inducers on stallion sperm capacitation and hyperactivation]Effect of human tubal fluid medium and hyperactivation inducers on stallion sperm capacitation and hyperactivation
Silva Jiménez, MauricioSmulders, Juan P.Guerra, MonserratValderrama, Ximena P.Letelier, ClaudiaAdams, Gregg P.Ratto, Marcelo H.[Cetrorelix suppresses the preovulatory LH surge and ovulation induced by ovulation-inducing factor (OIF) present in llama seminal plasma]Cetrorelix suppresses the preovulatory LH surge and ovulation induced by ovulation-inducing factor (OIF) present in llama seminal plasma

Ovulation-inducing factor (OIF) is a protein present in llama seminal plasma that has recently been identified as beta-Nerve Growth Factor (NGF) and it induces not only a high rate of ovulation but also appears to have luteotrophic properties in this species. A 2-by-2 experimental design was used...


Silva Jiménez, MauricioUlloa Leal, C.Norambuena, C.Fernandez, A.Adams, G. P.Ratto, Marcelo[Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter]Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter

The study was designed to compare the cleavage and blastocysts rate of in vitro matured alpaca and llama oocytes after chemical activation. Alpaca (n=90) and llama (n=85) ovaries were collected at a local slaughterhouse and transported within 2-3 h to the laboratory. Cumulus oocyte complexes (COC...


Ruiz, JaimeLandeo, LeandraMendoza, JoseCorrea, JorgeSilva Jiménez, MauricioRatto, Marcelo H.[In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation]In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation

We evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte-Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 degrees C, h...


Ruiz, JaimeSantayana, R. PauloMendoza, M. JoseLandeo, J. LeandraHuaman, ElizabethTicllacuri, FlamelMujica, L. FidelSilva Jiménez, MauricioRatto, Marcelo H.[Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas]Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

The development of assisted reproductive technologies such as embryo transfer (ET), artificial insemination (AI) and in vitro fertilization (IVF) in South American camelids is considerably behind that of other livestock species. Poor success of the embryo transfer technique has been related to a ...


Rano, Marcelo H.Silva Jiménez, MauricioHuanca, WilfredoHuanca, TeodosioAdams, Gregg P.[Induction of superovulation in South American camelids]Induction of superovulation in South American camelids

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