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The study was designed to compare the cleavage and blastocysts rate of in vitro matured alpaca and llama oocytes after chemical activation. Alpaca (n=90) and llama (n=85) ovaries were collected at a local slaughterhouse and transported within 2-3 h to the laboratory. Cumulus oocyte complexes (COC...


Ruiz, JaimeLandeo, LeandraMendoza, JoseCorrea, JorgeSilva Jiménez, MauricioRatto, Marcelo H.[In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation]In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation

We evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte-Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 degrees C, h...


Ruiz, JaimeSantayana, R. PauloMendoza, M. JoseLandeo, J. LeandraHuaman, ElizabethTicllacuri, FlamelMujica, L. FidelSilva Jiménez, MauricioRatto, Marcelo H.[Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas]Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

The ovulation-inducing effect of seminal plasma was first suggested in Bactrian camels over 30 years ago, initiating a long search to identify the 'ovulation-inducing factor' (OIF) present in camelids semen. During the last decade, primarily in llamas and alpacas, this molecule has been intensive...


Silva Jiménez, MauricioPaiva, LuisRatto, Marcelo H.[Ovulation mechanism in South American Camelids: The active role of beta-NGF as the chemical signal eliciting ovulation in llamas and alpacas]Ovulation mechanism in South American Camelids: The active role of beta-NGF as the chemical signal eliciting ovulation in llamas and alpacas

This study was designed to determine the effect of location of the preovulatory dominant follicle and stage of ovarian follicle development on ovulation rate and embryo survival in alpacas. In Experiment 1, mature lactating alpacas were randomly assigned to one of two groups according to the loca...


Ratto, MarceloCervantes, MiriamNorambuena Ardiles, MaríaSilva Jiménez, MauricioMiragaya, MarceloHuanca, Wilfredo[Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas]Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas

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