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This study investigated the genetic and antigenic diversity of Theileria parva in cattle from the Eastern and Southern zones of Tanzania. Thirty-nine (62%) positive samples were genotyped using 14 mini- and microsatellite markers with coverage of all four T. parva chromosomes. Wright's F index (F...


Elisa, M.Hasan, S.D.Moses, N.Elpidius, R.Skilton, Robert A.Gwakisa, P.S.[Genetic and antigenic diversity of Theileria parva in cattle in Eastern and Southern zones of Tanzania: A study to support control of East Coast fever]Genetic and antigenic diversity of Theileria parva in cattle in Eastern and Southern zones of Tanzania: A study to support control of East Coast fever
Kilalo, D.Ateka, E.M.Pappu, H.Skilton, Robert A.Harvey, Jagger J.W.[Identification of cowpea aphid borne mosaic virus in diseased passionfruit (Passiflora edulis Sims) and diagnostic test development]Identification of cowpea aphid borne mosaic virus in diseased passionfruit (Passiflora edulis Sims) and diagnostic test development
Wanjala, B.W.Muchugi, A.Obonyo, M.M.M.Harvey, Jagger J.W.Skilton, Robert A.Proud, J.Hanson, Jean[Genetic diversity of Napier Grass (Pennisetum purpureum Schumacher) germplasm based on amplified fragment length polymorphism]Genetic diversity of Napier Grass (Pennisetum purpureum Schumacher) germplasm based on amplified fragment length polymorphism

Theileriosis, caused by parasitic protozoa of the genus Theileria parasites, are among the major tick-borne diseases of ruminant livestock. The largest economic losses are attributed in particular to those caused by the leukoproliferative species of Theileria: T. parva, T. annulata and T. lestoqu...


Ali, A.M.Salih, Diaeldin A.Njahira, Moses N.Hassan, S.K.Hussein, A.M.E.Liu, Z.Yin, H.Pelle, RogerSkilton, Robert A.[Genotyping of Theileria lestoquardi from sheep and goats in Sudan to support control of Malignant Ovine Theileriosis]Genotyping of Theileria lestoquardi from sheep and goats in Sudan to support control of Malignant Ovine Theileriosis

African Cape buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (buffalo) has been r...


Bishop, Richard P.Hemmink, W.I.Morrison, J.D.Weir, W.Toye, Philip G.Sitt, T.Spooner, P.R.Musoke, A.J.Skilton, Robert A.Odongo, David O.[The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes]The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive fa...


Kang'ethe, Erastus K.Mulinge, E.K.Skilton, Robert A.Njahira, Moses N.Monda, J.G.Nyongesa, C.Mbae, C.K.Kamwati, S.K.[Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya]Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...


Nene, VishvanathLee, D.Quackenbush, J.Skilton, Robert A.Mwaura, S.Gardner, M.J.Bishop, Richard P.[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum

An improved Theileria parva DNA detection assay based on the polymerase chain reaction (PCR) using primers derived from the 104 kDa antigen (p104) gene was developed to detect parasite DNA in blood spots on filter paper. The specificity of the assay was validated using DNA from a wide range of ca...


Skilton, Robert A.Bishop, Richard P.Katende, J.M.Mwaura, S.Morzaria, S.P.[The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay]The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay

A cross-sectional study of serum antibody responses of cattle to tick-borne disease (TBD) parasites (Theileria parva, Theileria mutans, Anaplasma marginale and Babesia bigemina) was conducted on traditional smallholder mixed farms in Mbeere District in Kenya. The objective was to estimate the inf...


Gachohi, John M.Ngumi, P.N.Kitala, P.M.Skilton, Robert A.[Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya]Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya

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