The molecular typing and the indirect ELISA procedure were used to identify some alleles of favorable effect on the antibody response against the Newcastle disease virus (NDV). The NDV seroprevalence was 100.00% with 10.47% suspect and 89.63% positives. The protective level of ELISA antibody mean...
Hako Touko, B.A.
,
Keambou, T.C.
,
Han, J.M.
,
Bembide, C.
,
Cho, C.Y.
,
Skilton, Robert A.
,
Djikeng, Appolinaire
,
Ogugo, M.
,
Manjeli, Y.
,
Tebug Tumassang, T.
,
Zoli, P.A.
,
Osama, S.
,
[The Major Histocompatibility Complex B (MHC-B) and QTL Microsatellite Alleles of Favorable Effect on Antibody Response against the Newcastle Disease]
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The Major Histocompatibility Complex B (MHC-B) and QTL Microsatellite Alleles of Favorable Effect on Antibody Response against the Newcastle Disease
The objective of this study was to investigate the relationship between seroprevalence to Theileria parva infection in cattle and potential environmental and farm-level effects in 80 farms under traditional crop–livestock system in Mbeere District, Kenya. A standardized questionnaire was used to ...
Gachohi, John M.
,
Kitala, P.M.
,
Ngumi, P.N.
,
Skilton, Robert A.
,
[Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya]
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Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
,
Patel, S.P.
,
Skilton, Robert A.
,
Knowles, Donald P.
,
Scoles, Glen A.
,
Nene, Vishvanath
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata
The published genomic sequences of the two major host-transforming Theileria species of cattle represent a rich resource of information that has allowed novel bioinformatic and experimental studies into these important apicomplexan parasites. Since their publication in 2005, the genomes of T. ann...
Weir, W.
,
Sunter, J.
,
Chaussepied, M.
,
Skilton, Robert A.
,
Tait, A.
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
Shiels, B.
,
Langsley, G.
,
[Highly syntenic and yet divergent: a tale of two Theilerias]
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Highly syntenic and yet divergent: a tale of two Theilerias
Theileria parva causes East Coast fever, an economically important disease of cattle in sub-Saharan Africa. We describe a nested polymerase chain reaction (nPCR) assay for the detection of T. parva DNA in cattle blood spotted onto filter paper using primers derived from the T. parva-specific 104-...
Odongo, David O.
,
Sunter, J.D.
,
Kiara, Henry K.
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals]
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A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals
Vaccines based on recombinant Bm86 gut antigen from Boophilus microplus are a useful component of integrated control strategies against B. microplus infestations of cattle. The capacity of such vaccines to control heterologous infestations by two African tick species was investigated. The mean we...
Odongo, D.
,
Kamau, L.
,
Skilton, Robert A.
,
Mwaura, S.
,
Nitsch, C.
,
Musoke, A.J.
,
Taracha, E.A.
,
Daubenberger, C.A.
,
Bishop, Richard P.
,
[Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus]
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Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant mole...
Thekisoe, O.M.M.
,
Rambritch, N.E.
,
Nakao, R.
,
Bazie, R.S.
,
Mbati, P.
,
Namangala, B.
,
Malele, I.
,
Skilton, Robert A.
,
Jongejan, F.
,
Sugimoto, C.
,
Kawazu, S.I.
,
Inoue, N.
,
[Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes]
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Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
The genetic relationships among 192 superior, high-yielding, and disease-resistant sweetpotato [Ipomoea batatas (L.) Lam] accessions from the Ugandan germplasm collection were analyzed using 10 fluorescent labeled simple sequence repeat (SSR) markers. Relatedness among the genotypes was estimated...
The transcriptional control of gene expression is not well documented in the Arthropoda. We describe transcriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhipicephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of...
Kamau, L.
,
Skilton, Robert A.
,
Odongo, David O.
,
Mwaura, S.
,
Githaka, Naftaly W.
,
Kanduma, E.G.
,
Obura, M.
,
Kabiru, E.
,
Orago, A.
,
Musoke, A.J.
,
Bishop, Richard P.
,
[Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)]
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Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)
Although domestic cavies are widely used in sub-Saharan Africa as a source of meat and income, there are only a few studies of their population structure and genetic relatedness. This seminal study was designed with the main objective to assess the genetic diversity and determine the population s...
Ayagirwe, B.
,
Meutchieye, F.
,
Djikeng, Appolinaire
,
Skilton, Robert A.
,
Osama, S.
,
Manjeli, Y.
,
[Genetic diversity and structure of domestic cavy (Cavia porcellus) populations from smallholder farms in southern Cameroon]
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Genetic diversity and structure of domestic cavy (Cavia porcellus) populations from smallholder farms in southern Cameroon