A total of 202 poultry feed and its raw ingredients collected from different agroecological zones of Cameroon were examined for total mycoflora and the ability of A. flavus isolates to produces aflatoxin B1. Dilution plating was used for fungal isolation. The mean fungal contamination levels were...
Kana, J.R.
,
Gnonlonfin, G.J.B.
,
Harvey, Jagger J.W.
,
Wainaina, J.
,
Wanjuki, I.
,
Skilton, Robert A.
,
Teguia, A.
,
[Mycobiota and toxigenecity profile of Aspergillus flavus recovered from food and poultry feed mixtures in Cameroon]
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Mycobiota and toxigenecity profile of Aspergillus flavus recovered from food and poultry feed mixtures in Cameroon
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
,
Musoke, A.J.
,
Wells, C.W.
,
Yagi, Y.
,
Nene, Vishvanath
,
Spooner, P.R.
,
Gachanja, J.
,
Osaso, J.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Recently, mortalities among giraffes, attributed to infection with unique species of piroplasms were reported in South Africa. Although haemoparasites are known to occur in giraffes of Kenya, the prevalence, genetic diversity and pathogenicity of these parasites has not been investigated.
In thi...
Githaka, Naftaly W.
,
Konnai, S.
,
Skilton, Robert A.
,
Kariuki, E.
,
Kanduma, E.G.
,
Murata, S.
,
Ohashi, K.
,
[Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya]
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Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya
In this article, we review the epidemiology of East Coast fever (ECF), a tick borne infection of cattle, in Kenya. The major factors associated with epidemiology of ECF include the agro-ecological zone (AEZ), livestock production system (LPS) and both animal breed and age. These factors appear to...
Gachohi, John M.
,
Skilton, Robert A.
,
Hansen, F.
,
Ngumi, P.N.
,
Kitala, P.M.
,
[Epidemiology of East Coast fever (Theileria parva infection) in Kenya: Past, present and the future]
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Epidemiology of East Coast fever (Theileria parva infection) in Kenya: Past, present and the future
Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...
Morzaria, S.P.
,
Katende, J.
,
Musoke, A.J.
,
Nene, Vishvanath
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]
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Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...
Nene, Vishvanath
,
Lee, D.
,
Kang'a, S.
,
Skilton, Robert A.
,
Shah, Tushaar
,
Villiers, Etienne P. de
,
Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
,
Skilton, Robert A.
,
Katende, J.
,
Wells, C.W.
,
Nene, Vishvanath
,
McElwain, T.F.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
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Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...
Nene, Vishvanath
,
Lee, D.
,
Quackenbush, J.
,
Skilton, Robert A.
,
Mwaura, S.
,
Gardner, M.J.
,
Bishop, Richard P.
,
[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]
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AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Background
The ixodid tick Rhipicephalus appendiculatus transmits the apicomplexan protozoan parasite Theileria parva,which causes East coast fever (ECF), the most economically important cattle disease in eastern and southern Africa. Recent analysis of micro- and minisatellite markers showed an a...
Kanduma, E.G.
,
Mwacharo, Joram M.
,
Githaka, Naftaly W.
,
Kinyanjui, P.W.
,
Njuguna, J.
,
Kamau, L.M.
,
Kariuki, E.
,
Mwaura, S.
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya]
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Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya
Skilton, Robert A.
,
[Africa Biosciences Challenge Fund (ABCF): Empowering African scientists to solve Africa’s agricultural challenges]
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Africa Biosciences Challenge Fund (ABCF): Empowering African scientists to solve Africa’s agricultural challenges