Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geograp...
Kanduma, E.G.
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Mwacharo, Joram M.
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Mwaura, S.
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Njuguna, J.N.
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Nzuki, I.
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Kinyanjui, P.W.
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Githaka, Naftaly W.
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Heyne, H.
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Hanotte, Olivier H.
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Skilton, Robert A.
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Bishop, Richard P.
,
[Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya]
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Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
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Bishop, Richard P.
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Wells, C.W.
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Spooner, P.R.
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Gobright, E.I.
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Nkonge, C.
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Musoke, A.J.
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Macklin, M.D.
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Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)
In order to discriminate different bovine Theileria species and to characterise genetic diversity in the pathogenic T. parva, a range of diagnostic and molecular tools have been developed. The diagnostic tools are based on recombinant antigens used in ELISAs and the molecular tools are based on p...
Morzaria, S.P.
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Bishop, Richard P.
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Nene, Vishvanath
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Skilton, Robert A.
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Geysen, D.
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Mbogo, S.
,
[Molecular tools for characterisation of Theileria parva]
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Molecular tools for characterisation of Theileria parva
Tick salivary gland proteins are responsible for suppression/modulation of host immune responses and subsets of such genes whose expression is modified by Theileria parva infection would be candidates for inclusion in anti-tick/anti-pathogen combination vaccines. We used subtractive hybridisation...
Obura, M.
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Skilton, Robert A.
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Villiers, Etienne P. de
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Kutima, H.
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Bishop, Richard P.
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[Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva]
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Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva
Bishop, Richard P.
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Geysen, D.
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Skilton, Robert A.
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Odongo, D.
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Nene, Vishvanath
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Allsopp, B.A.
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Mbogo, S.
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Spooner, P.R.
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Morzaria, S.P.
,
[Genomic polymorphism, sexual recombination and molecular epidemiology of Theileria parva]
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Genomic polymorphism, sexual recombination and molecular epidemiology of Theileria parva
Mycotoxins affect poultry production by being present in the feed and directly causing a negative impact on bird performance. Carry-over rates of mycotoxins in animal products are, in general, small (except for aflatoxins in milk and eggs) therefore representing a small source of mycotoxins for h...
Kana, J.R.
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Gnonlonfin, G.J.B.
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Harvey, Jagger J.W.
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Wainaina, J.
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Wanjuki, I.
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Skilton, Robert A.
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Teguia, A.
,
[Assessment of aflatoxin contamination of maize, peanut meal and poultry feed mixtures from different agroecological zones in Cameroon]
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Assessment of aflatoxin contamination of maize, peanut meal and poultry feed mixtures from different agroecological zones in Cameroon
Molecular tools based on seminested RFLP-PCR techniques to characterize field parasites in bloodspots dried on filter paper permitted investigation of the extent and the dynamics of diversity of Theileria parva populaitons in the field. Parallel molecular studies explored the long-term genome sta...
Geysen, D.
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Bishop, Richard P.
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Skilton, Robert A.
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Dolan, T.T.
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Morzaria, S.P.
,
[Molecular epidemiology of Theileria parva in the field]
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Molecular epidemiology of Theileria parva in the field
Masembe, C.
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Michuki, G.
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Onyango, M.
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Rumberia, C.
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Norling M.
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Bishop, Richard P.
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Djikeng, Appolinaire
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Kemp, Stephen J.
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Orth, Alan
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Skilton, Robert A.
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Stahl, K.
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Fischer, A.
,
[Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus]
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Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus
Biological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick v...
Kanduma, E.G.
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Mwacharo, Joram M.
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Sunter, J.D.
,
Nzuk, I.
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Mwaura, S.
,
Kinyanjui, P.W.
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Kibe, M.
,
Heyne, H.
,
Hanotte, Olivier H.
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Skilton, Robert A.
,
Bishop, Richard P.
,
[Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus]
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Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus
Bramwel W.
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Wanjala, B.W.
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Obonyo, M.
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Wachira, F.N.
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Muchugi, A.
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Mulaa, M.
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Harvey, Jagger J.W.
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Skilton, Robert A.
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Proud, J.
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Hanson, Jean
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[Genetic diversity in Napier grass (Pennisetum purpureum) cultivars: Implications for breeding and conservation]
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Genetic diversity in Napier grass (Pennisetum purpureum) cultivars: Implications for breeding and conservation