A cross-sectional study of serum antibody responses of cattle to tick-borne disease (TBD) parasites (Theileria parva, Theileria mutans, Anaplasma marginale and Babesia bigemina) was conducted on traditional smallholder mixed farms in Mbeere District in Kenya. The objective was to estimate the inf...
Gachohi, John M.
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Ngumi, P.N.
,
Kitala, P.M.
,
Skilton, Robert A.
,
[Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya]
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Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya
The molecular typing and the indirect ELISA procedure were used to identify some alleles of favorable effect on the antibody response against the Newcastle disease virus (NDV). The NDV seroprevalence was 100.00% with 10.47% suspect and 89.63% positives. The protective level of ELISA antibody mean...
Hako Touko, B.A.
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Keambou, T.C.
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Han, J.M.
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Bembide, C.
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Cho, C.Y.
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Skilton, Robert A.
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Djikeng, Appolinaire
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Ogugo, M.
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Manjeli, Y.
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Tebug Tumassang, T.
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Zoli, P.A.
,
Osama, S.
,
[The Major Histocompatibility Complex B (MHC-B) and QTL Microsatellite Alleles of Favorable Effect on Antibody Response against the Newcastle Disease]
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The Major Histocompatibility Complex B (MHC-B) and QTL Microsatellite Alleles of Favorable Effect on Antibody Response against the Newcastle Disease
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
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Patel, S.P.
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Skilton, Robert A.
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Knowles, Donald P.
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Scoles, Glen A.
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Nene, Vishvanath
,
Villiers, Etienne P. de
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Bishop, Richard P.
,
[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata
Vaccines based on recombinant Bm86 gut antigen from Boophilus microplus are a useful component of integrated control strategies against B. microplus infestations of cattle. The capacity of such vaccines to control heterologous infestations by two African tick species was investigated. The mean we...
Odongo, D.
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Kamau, L.
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Skilton, Robert A.
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Mwaura, S.
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Nitsch, C.
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Musoke, A.J.
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Taracha, E.A.
,
Daubenberger, C.A.
,
Bishop, Richard P.
,
[Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus]
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Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
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Musoke, A.J.
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Wells, C.W.
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Yagi, Y.
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Nene, Vishvanath
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Spooner, P.R.
,
Gachanja, J.
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Osaso, J.
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Bishop, Richard P.
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Morzaria, S.P.
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[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Background
The ixodid tick Rhipicephalus appendiculatus transmits the apicomplexan protozoan parasite Theileria parva,which causes East coast fever (ECF), the most economically important cattle disease in eastern and southern Africa. Recent analysis of micro- and minisatellite markers showed an a...
Kanduma, E.G.
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Mwacharo, Joram M.
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Githaka, Naftaly W.
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Kinyanjui, P.W.
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Njuguna, J.
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Kamau, L.M.
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Kariuki, E.
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Mwaura, S.
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya]
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Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya
Bishop, Richard P.
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Musoke, A.J.
,
Skilton, Robert A.
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Morzaria, S.P.
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Gardner, M.
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Nene, Vishvanath
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[Theileria: Life cycle stages associated with the ixodid tick vector]
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Theileria: Life cycle stages associated with the ixodid tick vector
Commercial vaccines based on recombinant forms of the Bm86 tick gut antigen are used to control the southern cattle tick, Rhipicephalus microplus, a 1-host species, in Australia and Latin America. We describe herein sequence polymorphism in genes encoding Ra86 homologues of Bm86 in the brown ear ...
Kamau, L.M.
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Skilton, Robert A.
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Githaka, Naftaly W.
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Kiara, Henry K.
,
Kabiru, E.
,
Shah, Tushaar
,
Musoke, A.J.
,
Bishop, Richard P.
,
[Extensive polymorphism of Ra86 genes in field populations of Rhipicephalus appendiculatus from Kenya]
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Extensive polymorphism of Ra86 genes in field populations of Rhipicephalus appendiculatus from Kenya