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Journal Article 2015-12-30

Autor: Bishop, Richard P. Hemmink, W.I. Morrison, J.D. Weir, W. Toye, Philip G. Sitt, T. Spooner, P.R. Musoke, A.J. Skilton, Robert A. Odongo, David O.

African Cape buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (buffalo) has been r...

Enlace original: https://cgspace.cgiar.org/handle/10568/71030

Investigación en Agricultura
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Bishop, Richard P. ,  Hemmink, W.I. ,  Morrison, J.D. ,  Weir, W. ,  Toye, Philip G. ,  Sitt, T. ,  Spooner, P.R. ,  Musoke, A.J. ,  Skilton, Robert A. ,  Odongo, David O. ,  [The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes] ,  The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Journal Article 2002-03-15

Autor: Skilton, Robert A. Bishop, Richard P. Katende, J.M. Mwaura, S. Morzaria, S.P.

An improved Theileria parva DNA detection assay based on the polymerase chain reaction (PCR) using primers derived from the 104 kDa antigen (p104) gene was developed to detect parasite DNA in blood spots on filter paper. The specificity of the assay was validated using DNA from a wide range of ca...

Enlace original: https://cgspace.cgiar.org/handle/10568/21168

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Skilton, Robert A. ,  Bishop, Richard P. ,  Katende, J.M. ,  Mwaura, S. ,  Morzaria, S.P. ,  [The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay] ,  The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay
Journal Article 2010-07-01

Autor: Gachohi, John M. Ngumi, P.N. Kitala, P.M. Skilton, Robert A.

A cross-sectional study of serum antibody responses of cattle to tick-borne disease (TBD) parasites (Theileria parva, Theileria mutans, Anaplasma marginale and Babesia bigemina) was conducted on traditional smallholder mixed farms in Mbeere District in Kenya. The objective was to estimate the inf...

Enlace original: https://cgspace.cgiar.org/handle/10568/1447

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Gachohi, John M. ,  Ngumi, P.N. ,  Kitala, P.M. ,  Skilton, Robert A. ,  [Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya] ,  Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere District, Kenya
Journal Article 2011-01-15

Autor: Gachohi, John M. Kitala, P.M. Ngumi, P.N. Skilton, Robert A.

The objective of this study was to investigate the relationship between seroprevalence to Theileria parva infection in cattle and potential environmental and farm-level effects in 80 farms under traditional crop–livestock system in Mbeere District, Kenya. A standardized questionnaire was used to ...

Enlace original: https://cgspace.cgiar.org/handle/10568/2438

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Gachohi, John M. ,  Kitala, P.M. ,  Ngumi, P.N. ,  Skilton, Robert A. ,  [Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya] ,  Environment and farm factors associated with exposure to Theileria parva infection in cattle under traditional mixed farming system in Mbeere District, Kenya
Journal Article 2012-09-07

Autor: Gachohi, John M. Skilton, Robert A. Hansen, F. Ngumi, P.N. Kitala, P.M.

In this article, we review the epidemiology of East Coast fever (ECF), a tick borne infection of cattle, in Kenya. The major factors associated with epidemiology of ECF include the agro-ecological zone (AEZ), livestock production system (LPS) and both animal breed and age. These factors appear to...

Enlace original: https://cgspace.cgiar.org/handle/10568/21766

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Gachohi, John M. ,  Skilton, Robert A. ,  Hansen, F. ,  Ngumi, P.N. ,  Kitala, P.M. ,  [Epidemiology of East Coast fever (Theileria parva infection) in Kenya: Past, present and the future] ,  Epidemiology of East Coast fever (Theileria parva infection) in Kenya: Past, present and the future
Journal Article 1999

Autor: Morzaria, S.P. Katende, J. Musoke, A.J. Nene, Vishvanath Skilton, Robert A. Bishop, Richard P.

Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...

Enlace original: https://cgspace.cgiar.org/handle/10568/29383

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Morzaria, S.P. ,  Katende, J. ,  Musoke, A.J. ,  Nene, Vishvanath ,  Skilton, Robert A. ,  Bishop, Richard P. ,  [Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa] ,  Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa
Journal Article 1998

Autor: Katende, J. Morzaria, S.P. Toye, Philip G. Skilton, Robert A. Nene, Vishvanath Nkonge, C. Musoke, A.J.

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...

Enlace original: https://cgspace.cgiar.org/handle/10568/33099

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Katende, J. ,  Morzaria, S.P. ,  Toye, Philip G. ,  Skilton, Robert A. ,  Nene, Vishvanath ,  Nkonge, C. ,  Musoke, A.J. ,  [An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule] ,  An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Journal Article 2001

Autor: Bishop, Richard P. Geysen, D. Spooner, P.R. Skilton, Robert A. Nene, Vishvanath Dolan, T.T. Morzaria, S.P.

The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....

Enlace original: https://cgspace.cgiar.org/handle/10568/33253

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Bishop, Richard P. ,  Geysen, D. ,  Spooner, P.R. ,  Skilton, Robert A. ,  Nene, Vishvanath ,  Dolan, T.T. ,  Morzaria, S.P. ,  [Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle] ,  Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle
Journal Article 2017-08-01

Autor: Marcellino, Wani L. Salih, Diaeldin A. Njahira, Moses N. Ndiwa, Nicholas N. Araba, A. El Hussein, A.M. Seitzer, U. Ahmed, J.S. Bishop, Richard P. Skilton, Robert A.

A cross-sectional survey was carried out in four counties of Jonglei State, South Sudan, between May and June 2012 to determine the distribution and northern limit of Theileria parva, the causative agent of East Coast fever in cattle, and its tick vector Rhipicephalus appendiculatus, as a prerequ...

Enlace original: https://cgspace.cgiar.org/handle/10568/82990

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Marcellino, Wani L. ,  Salih, Diaeldin A. ,  Njahira, Moses N. ,  Ndiwa, Nicholas N. ,  Araba, A. ,  El Hussein, A.M. ,  Seitzer, U. ,  Ahmed, J.S. ,  Bishop, Richard P. ,  Skilton, Robert A. ,  [The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools] ,  The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools
Journal Article 2016-02-15

Autor: Kanduma, E.G. Mwacharo, Joram M. Mwaura, S. Njuguna, J.N. Nzuki, I. Kinyanjui, P.W. Githaka, Naftaly W. Heyne, H. Hanotte, Olivier H. Skilton, Robert A. Bishop, Richard P.

Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geograp...

Enlace original: https://cgspace.cgiar.org/handle/10568/69234

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Kanduma, E.G. ,  Mwacharo, Joram M. ,  Mwaura, S. ,  Njuguna, J.N. ,  Nzuki, I. ,  Kinyanjui, P.W. ,  Githaka, Naftaly W. ,  Heyne, H. ,  Hanotte, Olivier H. ,  Skilton, Robert A. ,  Bishop, Richard P. ,  [Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya] ,  Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya

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