Theileria parva causes an acute fatal disease in cattle, but infections are asymptomatic in the African buffalo (Syncerus caffer). Cattle can be immunized against the parasite by infection and treatment, but immunity is partially strain specific. Available data indicate that CD8+ T lymphocyte res...
Pelle, Roger
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Graham, S.P.
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Njahira, Moses N.
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Osaso, J.
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Saya, R.M.
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Odongo, David O.
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Toye, Philip G.
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Spooner, P.R.
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Musoke, A.J.
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Mwangi, D.M.
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Taracha, E.L.N.
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Morrison, W. Ivan
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Weir, W.
,
Silva, J.
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Bishop, Richard P.
,
[Two Theileria parva CD8 T Cell Antigen genes are more variable in buffalo than cattle parasites, but differ in pattern of sequence diversity]
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Two Theileria parva CD8 T Cell Antigen genes are more variable in buffalo than cattle parasites, but differ in pattern of sequence diversity
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
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Musoke, A.J.
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Wells, C.W.
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Yagi, Y.
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Nene, Vishvanath
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Spooner, P.R.
,
Gachanja, J.
,
Osaso, J.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...
Morzaria, S.P.
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Dolan, T.T.
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Norval, R.A.I.
,
Bishop, Richard P.
,
Spooner, P.R.
,
[Generation and characterisation of cloned Theileria parva parasites]
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Generation and characterisation of cloned Theileria parva parasites
Allsopp, B.A.
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Bayles, H.A.
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Allsopp, M.T.
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Cavalier-Smith, T.
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Bishop, Richard P.
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Carrington, D.M.
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Sohanpal, B.K.
,
Spooner, P.R.
,
[Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences]
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Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
Bishop, Richard P.
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Sohanpal, B.K.
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Kariuki, D.
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Young, A.S.
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Nene, Vishvanath
,
Baylis, H.
,
Allsopp, B.A.
,
Spooner, P.R.
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction
Irvin, A.D.
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Ocama, J.G.R.
,
Spooner, P.R.
,
[Cycle of bovine lymphoblastoid cells parasitized by Theileria parva]
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Cycle of bovine lymphoblastoid cells parasitized by Theileria parva
Theileria parva schizont-infected lymphocyte culture isolates from western, central and coastal Kenya were analysed for size polymorphism at 30 T. parva-specific variable number tandem repeat (VNTR) loci using a panel of mini- and micro-satellite markers. The mean number of alleles ranged from 3 ...
Odongo, David O.
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Oura, C.A.
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Spooner, P.R.
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Kiara, Henry K.
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Mburu, D.
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Hanotte, Olivier H.
,
Bishop, Richard P.
,
[Linkage disequilibrium between alleles at highly polymorphic mini- and micro-satellite loci of Theileria parva isolated from cattle in three regions of Kenya]
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Linkage disequilibrium between alleles at highly polymorphic mini- and micro-satellite loci of Theileria parva isolated from cattle in three regions of Kenya
Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species variable region, located bet...
Bishop, Richard P.
,
Allsopp, B.A.
,
Spooner, P.R.
,
Sohanpal, B.K.
,
Morzaria, S.P.
,
Gobright, E.I.
,
[Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences]
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Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences
Minami, T.
,
Spooner, P.R.
,
Irvin, A.D.
,
Ocama, J.G.R.
,
Dobbelaere, D.A.E.
,
Fujinaga, T.
,
[Characterisation of stocks of Theileria parva by monoclonal antibody profiles]
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Characterisation of stocks of Theileria parva by monoclonal antibody profiles