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Morzaria, S.P.Young, J.R.Spooner, P.R.Dolan, T.T.Bishop, Richard P.[Theileria parva: a restriction map and genetic recombination]Theileria parva: a restriction map and genetic recombination
Dobbelaere, D.A.E.Spooner, P.R.Barry, W.C.Irvin, A.D.[Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks]Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks

A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...


Morzaria, S.P.Dolan, T.T.Norval, R.A.I.Bishop, Richard P.Spooner, P.R.[Generation and characterisation of cloned Theileria parva parasites]Generation and characterisation of cloned Theileria parva parasites

A hybridoma cell line has been previously produced which secretes monoclonal antibodies that neutralize sporozoites of T. parva. Cells from this line were injected i/p into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily i/p injections of a m...


Dobbelaere, D.A.E.Spooner, P.R.[Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites]Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites

Mini- and microsatellite sequences show high levels of variation and therefore provide excellent tools for both the genotyping and population genetic analysis of parasites. Herein we describe the identification of a panel of 11 polymorphic microsatellites and 49 polymorphic minisatellites of th...


Oura, C.A.L.Odongo, David O.Lubega, G.W.Spooner, P.R.Tait, A.Bishop, Richard P.[A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva]A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva
Bishop, Richard P.Sohanpal, B.K.Kariuki, D.Young, A.S.Nene, VishvanathBaylis, H.Allsopp, B.A.Spooner, P.R.Dolan, T.T.Morzaria, S.P.[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction
Spooner, P.R.[Oxytetracycline inhibition of mitochondrial protein synthesis in bovine lymphocytes infected with Theileria parva or stimulated by mitogen]Oxytetracycline inhibition of mitochondrial protein synthesis in bovine lymphocytes infected with Theileria parva or stimulated by mitogen

Humoral immune responses to schizont antigens from six stocks of Theileria parva were compared by two-dimensional Western blotting using sera from cattle that had been infected with a T. parva stock or a clone. Isoelectric points of a polymorphic immunodominant molecule (PIM) of schizonts that in...


Kishima, M.Dolan, T.T.Njamunggeh, R.E.G.Nkonge, C.G.Spooner, P.R.[Humoral immune responses to Theileria parva in cattle as measured by two-dimensional Western blotting]Humoral immune responses to Theileria parva in cattle as measured by two-dimensional Western blotting

A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isol...


Bishop, Richard P.Spooner, P.R.Sohanpal, B.K.[Genetic fingerprinting of Theileria parva using a telomeric DNA probe]Genetic fingerprinting of Theileria parva using a telomeric DNA probe

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