Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
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Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...
Bishop, Richard P.
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Gobright, E.I.
,
Spooner, P.R.
,
Allsopp, B.A.
,
Sohanpal, B.K.
,
Collins, N.
,
[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]
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Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva
Irvin, A.D.
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Ocama, J.G.R.
,
Spooner, P.R.
,
[Cycle of bovine lymphoblastoid cells parasitized by Theileria parva]
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Cycle of bovine lymphoblastoid cells parasitized by Theileria parva
Morzaria, S.P.
,
Irvin, A.D.
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Taracha, E.A.
,
Spooner, P.R.
,
Voigt, W.P.
,
Fujinaga, T.
,
Katende, J.M.
,
[Immunization against East Coast fever. The use of selected stocks of Theileria parva for immunization of cattle exposed to field challenge]
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Immunization against East Coast fever. The use of selected stocks of Theileria parva for immunization of cattle exposed to field challenge
Morzaria, S.P.
,
Spooner, P.R.
,
Bishop, Richard P.
,
Musoke, A.J.
,
Young, J.R.
,
[SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis]
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SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis
The preparation of Theileria parva stabilates has been described by Cunningham et al (1973). The method is detailed in the FAO report (1978) and the FAO tick and tick-borne Disease Manual (1984). Theileria parva stabilates are a source of viable sporozoites that can be used to immunise cattle by ...
Morzaria, S.P.
,
Spooner, P.R.
,
Bishop, Richard P.
,
Mwaura, S.
,
[The preparation of a composite stabilate for immunisation against East Coast fever]
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The preparation of a composite stabilate for immunisation against East Coast fever
Theileria parva schizont-infected lymphocyte culture isolates from western, central and coastal Kenya were analysed for size polymorphism at 30 T. parva-specific variable number tandem repeat (VNTR) loci using a panel of mini- and micro-satellite markers. The mean number of alleles ranged from 3 ...
Odongo, David O.
,
Oura, C.A.
,
Spooner, P.R.
,
Kiara, Henry K.
,
Mburu, D.
,
Hanotte, Olivier H.
,
Bishop, Richard P.
,
[Linkage disequilibrium between alleles at highly polymorphic mini- and micro-satellite loci of Theileria parva isolated from cattle in three regions of Kenya]
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Linkage disequilibrium between alleles at highly polymorphic mini- and micro-satellite loci of Theileria parva isolated from cattle in three regions of Kenya
The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than i...
Patel, Ekta
,
Lubembe, D.M.
,
Gachanja, J.
,
Mwaura, S.
,
Spooner, P.R.
,
Toye, Philip G.
,
[Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity]
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Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity
Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species variable region, located bet...
Bishop, Richard P.
,
Allsopp, B.A.
,
Spooner, P.R.
,
Sohanpal, B.K.
,
Morzaria, S.P.
,
Gobright, E.I.
,
[Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences]
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Theileria: Improved species discrimination using oligonucleotide derived from large subunit ribosomal RNA sequences