We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...
Suarez, MC
,
Bernal, A.
,
Gutiérrez, J.
,
Tohme, Joseph M.
,
Fregene, Martin A.
,
[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]
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Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)
Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...
Fregene, Martin A.
,
Okogbenin, Emmanuel
,
Mba, C
,
Angel, F.
,
Suarez, MC
,
Gutiérrez A., Janeth Patricia
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Bonierbale, Merideth W.
,
Tohme, Joseph M.
,
[Genome mapping in cassava improvement: Challenges, achievements and opportunities]
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Genome mapping in cassava improvement: Challenges, achievements and opportunities
Cassava (Manihot esculenta) is an allogamous, vegetatively propagated, Neotropical crop that is also widely grown in tropical Africa and Southeast Asia. To elucidate genetic diversity and differentiation in the crop's primary and secondary centers of diversity, and the forces shaping them, SSR ma...
Fregene, Martin A.
,
Suarez, MC
,
Mkumbira, J.
,
Kulembeka, H.P.
,
Ndedya, E
,
Kulaya, A
,
Mitchel, S
,
Gullberg, U
,
Rosling, H.
,
Dixon, Alfred G.O.
,
Dean, R.
,
Kresovich, S
,
[Simple sequence repeat marker diversity in cassava landraces: Genetic diversity and differentiation in an asexually propagated crop]
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Simple sequence repeat marker diversity in cassava landraces: Genetic diversity and differentiation in an asexually propagated crop