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Objectives: The objective of this study was to assess whether cytotoxic T cells (CTL) generated by the live vaccine, known as “ITM Muguga cocktail”, which is used for the cattle disease East Cost fever (ECF) in Sub-Saharan Africa, showed a broad reactivity against many different strains of the ca...


Steinaa, LucillaSvitek, NicholasAwino, EliasSaya, RosemaryToye, Philip G.[Cytotoxic T lymphocytes from cattle sharing the same MHC class I haplotype and immunized with live Theileria parva sporozoites differ in antigenic specificity]Cytotoxic T lymphocytes from cattle sharing the same MHC class I haplotype and immunized with live Theileria parva sporozoites differ in antigenic specificity

Tremendous progress has been made over the last ten years on East Coast fever (ECF) research. Publication of a reference genome sequence of Theileria parva, the causative agent of ECF, has led to a more thorough characterization of the genotypic and antigenic diversity of the pathogen. It also fa...


Nene, VishvanathKiara, Henry K.Lacasta, AnnaPelle, RogerSvitek, NicholasSteinaa, Lucilla[The biology of Theileria parva and control of East Coast fever—Current status and future trends]The biology of Theileria parva and control of East Coast fever—Current status and future trends

Svitek, NicholasSaya, R.Awino, EliasGilbert, S.C.Poole, Elizabeth J.Nene, VishvanathSteinaa, Lucilla[Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination]Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination

Kiara, Henry K.Steinaa, LucillaSvitek, NicholasSchieck, Elise G.Toye, Philip G.[Access to livestock health interventions and products in dairy and cattle value chains in Tanzania]Access to livestock health interventions and products in dairy and cattle value chains in Tanzania
Svitek, NicholasSaya, R.Awino, EliasMunyao, S.Njoroge, T.Gilbert, S.Nene, VishvanathSteinaa, Lucilla[Evaluating the Ad5/MVA antigen delivery systems]Evaluating the Ad5/MVA antigen delivery systems

We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...


Svitek, NicholasAwino, EliasNene, VishvanathSteinaa, Lucilla[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva

Awino, EliasAye, R.Bertrand, S.Gesharisha, J.Gicheru, N.Jores, JoergLiljander, Anne M.Muriuki, C.Naessens, JanNene, VishvanathNjoroge, T.Nyongesa, S.Pelle, RogerSsajjakambwe, P.Saya, R.Schieck, Elise G.Steinaa, LucillaSvitek, NicholasWasilwa, J.[ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)]ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)

Bett, Bernard K.Gachohi, John M.Karungo, C.Bukachi, S.Svitek, NicholasNjenga, K.Oyas, H.[Towards the development of optimal vaccination strategies for Rift Valley fever in East Africa]Towards the development of optimal vaccination strategies for Rift Valley fever in East Africa

Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradien...


Svitek, NicholasTaracha, E.L.N.Saya, R.Awino, EliasNene, VishvanathSteinaa, Lucilla[Analysis of the cellular immune responses to vaccines]Analysis of the cellular immune responses to vaccines

Svitek, Nicholas[Immunoinformatics and reverse vaccinology, potential application to development of an ASF vaccine]Immunoinformatics and reverse vaccinology, potential application to development of an ASF vaccine

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