In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeri...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Panis, B.
,
Cammue, B.P.
,
Volckaert, G.
,
Swennen, R.
,
[Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions]
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Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions
Shoot cultures of 401 banana clones were conserved under slow growth conditions (16±1°C, 25μmol m−2 s−1). Storage duration-defined as 60% survival time of 20 shoot cultures of a clone-averaged 334 days. However, large differences occurred among the different genomic (sub)groups and even within th...
Houwe, I. van den
,
Smet, K. de
,
Tezenas du Montcel, H.
,
Swennen, R.
,
[Variability in storage potential of banana shoot cultures under medium term storage conditions]
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Variability in storage potential of banana shoot cultures under medium term storage conditions
The term banana is meant throughout this chapter to cover dessert and cooking bananas as well as plantains. With an estimated total production of more than 76 million tons in 1992 (FAO 1993), banana is the no. 1 fruit crop in the world. Ninety-nine percent of world's annual production is cultivat...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Swennen, R.
,
Panis, B.
,
[Genetic transformation in Musa species (Banana)]
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Genetic transformation in Musa species (Banana)
Bananas are important staples in tropical and sub-tropical regions and their potential as a source of provitamin A has recently attracted attention for biofortification. A collection of 189 banana genotypes (AAB-plantains, M. acuminata cultivars and bred hybrids) was screened to determine variabi...
Amah, D.
,
Alamu, E.O.
,
Adesokan, M.
,
Biljon, A. van
,
Maziya-Dixon, B.B.
,
Swennen, R.
,
Labuschagne, M.
,
[Variability of carotenoids in a Musa germplasm collection and implications for provitamin A biofortification]
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Variability of carotenoids in a Musa germplasm collection and implications for provitamin A biofortification
Vuylsteke, D.R.
,
Swennen, R.
,
Langhe, E. de
,
[Tissue culture technology for the improvement of Africa plantains]
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Tissue culture technology for the improvement of Africa plantains
Tissue culture is increasingly being used as a vehicle for the safe exchange of Musa germplasm. However, certain pathogens, e.g banana bunchy-top virus (BBTV), may pass undetected through in vitro culture. To meet the urgent need for introducing new desired germplasm for Musa breeding and reduced...
Vuylsteke, D.R.
,
Schoofs, J.
,
Swennen, R.
,
Adejare, G.
,
Ayodele, M.
,
Langhe, E. de
,
[Shoottip culture and thirdcountry quarantine to facilitate the introduction of new Musa germplasm into West Africa]
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Shoottip culture and thirdcountry quarantine to facilitate the introduction of new Musa germplasm into West Africa
Hahn, S.K.
,
Vuylsteke, D.R.
,
Swennen, R.
,
[First reactions to ABB cooking bananas distributed in southeastern Nigeria]
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First reactions to ABB cooking bananas distributed in southeastern Nigeria
Banana and plantain (Musa spp.) are the fourth largest food commodity after rice, wheat, and milk in terms of the gross value of production (CGIAR 1992). They are produced in the humid tropics of many developing economies; about 74 million tons are produced annually (FAO 1991).
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...
Sági, L.
,
Panis, B.
,
Remy, S.
,
Schoofs, H.
,
Smet, K. de
,
Swennen, R.
,
Cammue, B.P.
,
[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]
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Genetic transformation of banana and plantain (Musa spp.) via particle bombardment
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...