Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes...
Šimoníková, D.
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Němečková, A.
,
Karafiátová, M.
,
Uwimana, B.
,
Swennen, R.
,
Doležel, Jaroslav
,
Hřibová, E.
,
[Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)]
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Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)
East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into th...
Nemecková, A.
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Christelová, P.
,
Cížková, J.
,
Nyine, M.
,
Houwe, I. van den
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Svacina, R.
,
Uwimana, B.
,
Swennen, R.
,
Doležel, Jaroslav
,
Hribova, E.
,
[Molecular and cytogenetic study of east African highland banana]
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Molecular and cytogenetic study of east African highland banana
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...