T cells bearing the gamma delta antigen receptor (gamma delta T cells) can constitute up to 50 percent of T cells in the peripheral blood and lymphoid organs of young cattle. We present data showing that gamma delta T cells are involved in immune responses against Theileria parva. Gamma delta T c...
Daubenberger, C.A.
,
Taracha, E.L.N.
,
Gaidulis, L.
,
Davis, W.C.
,
McKeever, Declan J.
,
[Bovine gamma delta T cell responses to the intracellular protozoan parasite Theileria parva]
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Bovine gamma delta T cell responses to the intracellular protozoan parasite Theileria parva
To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p...
Honda, Y.
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Waithaka, M.M.
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Taracha, E.L.N.
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Duchateau, L.
,
Musoke, A.J.
,
McKeever, Declan J.
,
[Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection]
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Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection
Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated through the destruction of parasite infected lymphocytes by MHC class I restricted CD8+ CTL. Six parasite proteins targeted by CTL from T. parva immune cattle have been identified and raised...
Graham, S.P.
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Pelle, Roger
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Villiers, Etienne P. de
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Yamage, M.
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Mwangi, D.M.
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Honda, Y.
,
Ellis, S.A.
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MacHugh, Niall D.
,
Morrison, W. Ivan
,
Taracha, E.L.N.
,
[Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva]
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Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva
Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...
Graham, S.P.
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Saya, R.
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Awino, Elias
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Ngugi, D.
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Nyanjui, J.K.
,
Hecker, R.
,
Taracha, E.L.N.
,
Nene, Vishvanath
,
[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]
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Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...
Ballingall, K.T.
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Mwangi, D.M.
,
MacHugh, Niall D.
,
Taracha, E.L.N.
,
Totte, P.
,
McKeever, Declan J.
,
[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]
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A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
There is strong evidence that class I MHC-restricted parasite-specific CD8+ CTL protect cattle against the protozoan parasite Theileria parva. As part of an effort to develop a subunit vaccine for the induction of these responses, we have investigated the factors involved in the generation of T. ...
Howard, C.J.
,
Sopp, P.
,
Parsons, K.R.
,
McKeever, Declan J.
,
Taracha, E.L.N.
,
Jones, B.V.
,
MacHugh, Niall D.
,
Morrison, W. Ivan
,
[Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45]
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Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45
Heterologous priming-boosting vaccination regimens involving priming with plasmid DNA antigen constructs and inoculating (boosting) with the same recombinant antigen expressed in replication-attenuated poxviruses have recently been demonstrated to induce immunity, based on CD4(+)- and CD8(+)-T-c...