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In a study Bovine DNA was prepared from a Theileria parva-transformed lymphobastoid cell line derived from the PBM of an heterozygons Boran (Bos indicus) steer. It was observed that linkage between the bovine DR alpha and DR beta subregions on the basis that a 1-2 Mbp Sfi1 and a 0.6 Mbp not 1 fra...
Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...
The recent amalgamation of ILRAD and ILCA provides the perfect platform for an African livestock genetic characterization program. The knowledge accumulated at ILCA over the years by its scientists, their network of contacts and collaborations, and the network of contacts and collaborations estab...
The C57BL/6 BALB/c and A/J inbred laboratory mouse strains often express different response phenotypes when challenged with a variety of infectious agents (Morrison et al. 1978; Bradley 1977; Hormmaeche et al. 1985). In view of the pivotal role of tumor necrosis factor Tnfa in innate immunity, we...
The tumor necrosis factor (TNF) locus comprises the tandemly arranged genes coding for Tnfa (cachectin) and Tnfb (lymphotoxin-alpha) (Semon et al 1987). In mice, the TNF locus is located on chromosome 17 (MMU17) in the major histocompatibility complex (MHC) region. Tnfa and soluble lymphotoxin al...
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