In a study Bovine DNA was prepared from a Theileria parva-transformed lymphobastoid cell line derived from the PBM of an heterozygons Boran (Bos indicus) steer. It was observed that linkage between the bovine DR alpha and DR beta subregions on the basis that a 1-2 Mbp Sfi1 and a 0.6 Mbp not 1 fra...
Bensaid, A.M.
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Young, J.R.
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Kaushal, A.
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Teale, A.J.
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[Genomic organization of the bovine class II MHC studied with field inversion gel electrophoresis]
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Genomic organization of the bovine class II MHC studied with field inversion gel electrophoresis
Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...
Wambugu, J.
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Kanguha, E.
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Hanotte, Olivier H.
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Davis, S.
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Taylor, J.
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Skow, L.
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Brunner, R.
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Schwerin, M.
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Teale, A.J.
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Iraqi, F.A.
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[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]
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Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
The recent amalgamation of ILRAD and ILCA provides the perfect platform for an African livestock genetic characterization program. The knowledge accumulated at ILCA over the years by its scientists, their network of contacts and collaborations, and the network of contacts and collaborations estab...
Hanotte, Olivier H.
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Teale, A.J.
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[New international livestock institute applies molecular genetics to African livestock biodiversity conservation and breeding]
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New international livestock institute applies molecular genetics to African livestock biodiversity conservation and breeding
Toye, Philip G.
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Teale, A.J.
,
[Gene transfection in mammalian cells and its application in studies of immunity to East Coast fever]
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Gene transfection in mammalian cells and its application in studies of immunity to East Coast fever
The C57BL/6 BALB/c and A/J inbred laboratory mouse strains often express different response phenotypes when challenged with a variety of infectious agents (Morrison et al. 1978; Bradley 1977; Hormmaeche et al. 1985). In view of the pivotal role of tumor necrosis factor Tnfa in innate immunity, we...
Iraqi, F.A.
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Teale, A.J.
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[Cloning and sequencing of the Tnfa genes of three inbred mouse strains]
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Cloning and sequencing of the Tnfa genes of three inbred mouse strains
The tumor necrosis factor (TNF) locus comprises the tandemly arranged genes coding for Tnfa (cachectin) and Tnfb (lymphotoxin-alpha) (Semon et al 1987). In mice, the TNF locus is located on chromosome 17 (MMU17) in the major histocompatibility complex (MHC) region. Tnfa and soluble lymphotoxin al...
Iraqi, F.A.
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Teale, A.J.
,
[Polymorphism in the microsatellite located in the promoters of the Tnfa and Tnfb genes of different mouse strains]
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Polymorphism in the microsatellite located in the promoters of the Tnfa and Tnfb genes of different mouse strains
In view of the Tnfa gene location within the major histocompatibility (MHC) region and reports of its importance in disease protection activity, this paper examines the TNF a genes of a number of different mouse strains which were known to be of different disease response phenotypes. Twelve inbre...
Iraqi, F.A.
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Teale, A.J.
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[Polymorphisms, in the Tnfa gene of different inbred mouse strains]
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Polymorphisms, in the Tnfa gene of different inbred mouse strains
The unique potential of biotechnology to provide new solutions to old problems constraining the contribution of livestock to farming systems in the developing world is emphasized in this paper. An overview of biotechnological aspects of livestock research within the CGIAR, including a description...
Teale, A.J.
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[Biotechnology: A key element in the CGIAR's livestock research programme]
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Biotechnology: A key element in the CGIAR's livestock research programme
Kemp, Stephen J.
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Teale, A.J.
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[An assay for the fully automated reading of the lymphocytotoxicity test and its application to the detection of bovine class I and class II MHC antigens]
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An assay for the fully automated reading of the lymphocytotoxicity test and its application to the detection of bovine class I and class II MHC antigens