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Several applications of biotechnology have been successfully used recently in cowpea. A molecular map of cowpea has been constructed using RFLP markers, and this has already facilitated the localization of certain quantitative trait loci; gene chromosome localization using in situ hybridization i...


Monti, L.Murdock, L.Thottappilly, G.[Oppotunities for biotechnology in cowpea]Oppotunities for biotechnology in cowpea

Cowpea crops are susceptible low more than 20 viral diseases. Some of the most destructive viral pathogens are transmitted from one plant generation to the next through the seed, and thus are generally disseminated to most cowpea-producing regions of the world. Seedborne cowpea viruses, after est...


Hampton, R.Thottappilly, G.Rossel, H.[Viral diseases of cowpea and their control by resistance conferring genes]Viral diseases of cowpea and their control by resistance conferring genes

The effect of temperature on symptom expression and detection of banana streak badnavirus (BSV) by immunosorbent electronmicroscopy (ISEM) and enzyme-linked immunosorbent assay of 12 in vitro-propagated plantain hybrids (genome AAB × AA), 3 ABB cooking banana, and 3 AAB plantain landraces was stu...


Dahal, G.Hughes, J.Thottappilly, G.Lockhart, B.E.L.[Effect of temperature on symptom expression and reliability of banana streak badnavirus detection in naturally infected plantain and banana (Musa spp.)]Effect of temperature on symptom expression and reliability of banana streak badnavirus detection in naturally infected plantain and banana (Musa spp.)

Five potyvirus isolates from various cowpea-producing areas (Guinea and Sudan savannas and forest zones) in Nigeria were identified by serology and infectivity tests as BlCMV (Isolates IT15, IT16 and WC) or as CAMV (isolates IT11 and ZARIA) and compared with the standard isolate of blackeye cowpe...


Gumedzoe, M.Y.Rossel, H.W.Thottappilly, G.Asselin, A.Huguenot, C.[Reaction of cowpea (Vigna unguiculata L. Walp.) to six isolates of blackeye cowpea mosaic virus (BlCMV) and cowpea aphidborne mosaic virus (CAMV), two potyviruses infecting cowpea in Nigeria]Reaction of cowpea (Vigna unguiculata L. Walp.) to six isolates of blackeye cowpea mosaic virus (BlCMV) and cowpea aphidborne mosaic virus (CAMV), two potyviruses infecting cowpea in Nigeria

Knowledge of the genetic relationships and variability among cultivated germplasm is important for facilitating the transfer of useful genes and maximizing the use of available germplasm resources. In this study, random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversit...


Mignouna, H.D.Ng, N.Q.Ikea, J.Thottappilly, G.[Genetic diversity in cowpea as revealed by random amplified polymorphic DNA]Genetic diversity in cowpea as revealed by random amplified polymorphic DNA

(ACMV) were assessed in shoots regenerated from single-node cuttings of three cassava genotypes differing in resistance to ACMV. Plants of the moderately resistant TMS 4(2)1425 and susceptible TMS 60506 did not recover from the disease as rapidly as those of the resistant TMS 30001, Recovery was ...


Njock, T.E.Atiri, G.I.Thottappilly, G.Thresh, J.M.[Disease development and recovery in resistant and susceptible cassava genotypes infected by African cassava mosaic geminivirus]Disease development and recovery in resistant and susceptible cassava genotypes infected by African cassava mosaic geminivirus

The effect of mixed infection of cowpea aphid-borne mosaic (CAMV) and cucumber mosaic (CMV) viruses on cowpea yield varied with the resistance levels of cowpea lines, since there was a strong interaction between the latter and virus infection types atP ≤ 0.01. In cowpea line TVu 3629, with low re...


Mih, A.M.Atiri, G.I.Thottappilly, G.[Relationships between coinfection with cowpea aphidborn mosaic and cucumber mosaic viruses and yield of cowpea lines with varying resistance to these viruses]Relationships between coinfection with cowpea aphidborn mosaic and cucumber mosaic viruses and yield of cowpea lines with varying resistance to these viruses
Mignouna, H.Asiedu, RobertThottappilly, G.Hahn, S.K.[Genetic diversity and phylogenetic relationships in the genus Dioscorea using PCRRAPD]Genetic diversity and phylogenetic relationships in the genus Dioscorea using PCRRAPD

The survivorship, fecundity and development time (egg to adult) of Cicadulina storeyi China, C. arachidis China and C. ghaurii Dabrowski were studied at three constant temperatures (20, 25 and 30 .C). Additional tests were conducted to descriptionbe maize streak virus (MSV) transmission by C. ara...


Asanzi, M.C.Bosque Pérez, N.A.Nault, L.R.Gordon, D.T.Thottappilly, G.[Biology of Cicadulina species (Homoptera: Cicadellidae) and transmission of maize streak virus]Biology of Cicadulina species (Homoptera: Cicadellidae) and transmission of maize streak virus

Meristem-tip culture is being used to eliminate viruses from plants. The fact that some viruses are easier to eliminate than others has been utilized in thermotherapy and chemotherapy, along with meristem-tip culture, to increase the efficacy of disease elimination. Tissue culture materials are c...


Ng, S.Y.C.Rossel, H.W.Thottappilly, G.[The role of tissue culture in the establishment of disease free germplasm for international distribution]The role of tissue culture in the establishment of disease free germplasm for international distribution

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