In a survey for cassava mosaic begomoviruses conducted in 1997 and 1998 in Nigeria, East African cassava mosaic virus (EACMV) was detected by the polymerase chain reaction together with African cassava mosaic virus (ACMV) in 27 out of 290 cassava leaf samples of infected plants from 254 farmers' ...
Ogbe, F.O.
,
Thottappilly, G.
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Dixon, Alfred G.O.
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Atiri, G.I.
,
Mignouna, H.D.
,
[Variants of East African cassava mosaic virus and its distribution in double infections with African cassava mosaic virus in Nigeria]
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Variants of East African cassava mosaic virus and its distribution in double infections with African cassava mosaic virus in Nigeria
In a study to map out the genes confering resistanceto African cassava msaic disease (ACMD), a cost-effective protocol for zygotic embryo culture developed at the International Institute of Tropical Agriculture(IITA) using 1/2 strenght Murashige and Skoog's basal media, was employed to germinatet...
Thottappilly, G.
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Ng, N.Q.
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Rossel, H.W.
,
[Screening germplasm of Vigna vexillata for resistance to cowpea mottle virus]
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Screening germplasm of Vigna vexillata for resistance to cowpea mottle virus
Deux virus du niébé (Vigna unguiculata), le virus de la marbrure du niébé et le virus de la mosaïque jaune du niébé, ont été étudiés. Les deux virus ont été identifiés et caractérisés à l'aide de la gamme de plantes indicatrices et de méthodes sérologiques. Les deux virus sont très répandus dans ...
Gumedzoe, M.Y.
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Sunu, D.Y.
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Thottappilly, G.
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Asselin, A.
,
[Importance du virus de la marbure du niebe et du virus de la mosaique jaune du niebe au Togo]
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Importance du virus de la marbure du niebe et du virus de la mosaique jaune du niebe au Togo
The concentration of African cassava mosaic virus (ACMV) was assessed by enzyme-linked immunosorbent assay in relation to symptom severity among resistant, moderately resistant and susceptible cassava genotypes. Resistant genotype NR 8083 had significantly lower symptom severity scores (P < 0·05)...
Ogbe, F.
,
Atiri, G.
,
Dixon, Alfred G.O.
,
Thottappilly, G.
,
[Symtom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes]
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Symtom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes
A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced ...
Njukeng, A.P.
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Ariri, G.I.
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Hughes, Jacqueline d'Arros
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Agindoran, B.O.
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Mignouna, H.D.
,
Thottappilly, G.
,
[A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.]
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A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
Reliable characterization of the variation among wild and cultivated yams in Nigeria is essential for improved management and efficient utilization of yam genetic resources. RAPD and double stringency PCR (DS-PCR) analyses were used to investigate genetic relationships and the extent of redundanc...
Mignouna, H.
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Abang, Mathew M.
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Wanyera, N.
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Chikaleke, V.
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Asiedu, Robert
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Thottappilly, G.
,
[PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation]
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PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation
Vigna vexillata is a wild cross-incompatible relative of cowpea. It is highly resistant to several diseases and pests plaguing cowpea. A linkage map was developed for V. vexillata comprising 120 markers, including 70 random amplified polymorphic DNAs, 47 amplified fragment length polymorphisms, o...
Ogundiwin, E.
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Thottappilly, G.
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Akenova, M.E.
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Pillay, M.
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Fatokun, C.A.
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[A genetic linkage map of Vigna vexillata]
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A genetic linkage map of Vigna vexillata
A survey was carried out in the 1996/97 and 1997/98 growing seasons on a field planted in three replicates with five clones of cassava at the International Institute of Tropical Agriculture, Ibadan, located in a transition forest, to determine the effects of cassava genotype and climate on the de...
Abdullahi, I.
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Atiri, G.I.
,
Dixon, Alfred G.O.
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Winter, S.
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Thottappilly, G.
,
[Effects of cassava genotype, climate, and the Bemisia tabaci vector population on the development of African cassava mosaic geminivirus (ACMV)]
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Effects of cassava genotype, climate, and the Bemisia tabaci vector population on the development of African cassava mosaic geminivirus (ACMV)
Maize (Zea mays) and itch grass (Rottboellia cochinchinensis) plants exhibiting a mild mosaic or mottle were collected from a farmer's field near Mokwa, Nigeria, in 1993. Icosahedral virions (approximately 28 to 30 nm) were purified from symptomatic tissue by differential centrifugation in 0.1 M ...
Thottappilly, G.
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Qiu, W.P.
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Batten, J.S.
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Hughes, J.N.
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Scholthof, K.B.
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[A new virus on maize in Nigeria: maize mild mottle virus]
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A new virus on maize in Nigeria: maize mild mottle virus