Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Ramírez, H.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
Tohme, Joseph M.
,
López, Y.
,
[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético
Ballén-Taborda, Carolina
,
Plata, Germán
,
Ayling, Sarah
,
Rodríguez-Zapata, Fausto
,
Becerra López Lavelle, Luis Augusto
,
Duitama, Jorge
,
Tohme, Joseph M.
,
[Identification of Cassava MicroRNAs under abiotic stress]
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Identification of Cassava MicroRNAs under abiotic stress
Vargas, J.
,
Tohme, Joseph M.
,
Debouck, Daniel G.
,
[Common bean domestication in the southern Andes]
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Common bean domestication in the southern Andes
Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...
Fajardo, Diego
,
Angel, F.
,
Grum, M.
,
Tohme, Joseph M.
,
Lobo Arias, M
,
Roca, William M.
,
Sánchez, I.
,
[Genetic variation analysis of the genus Passiflora L. using RAPD markers]
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Genetic variation analysis of the genus Passiflora L. using RAPD markers
Montes, L
,
Azudia, CD
,
Buitrago, C.
,
Debouck, Daniel G.
,
Tohme, Joseph M.
,
Fregene, Martin A.
,
[Simple sequence repeat (SSR) assessment of genetic diversity of local cassava varieties from Guatemala]
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Simple sequence repeat (SSR) assessment of genetic diversity of local cassava varieties from Guatemala
Giraldo Pasmín, O.X.
,
Quintero, C.
,
Plata, G
,
Rodríguez, F.
,
Borrero Correa, Jaime C.
,
Martínez Racines, César Pompilio
,
Tohme, Joseph M.
,
[Identification of SNPs markers for biofortification in rice]
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Identification of SNPs markers for biofortification in rice
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × C...
Jorge, V
,
Fregene, Martin A.
,
Duque E., Myriam Cristina
,
Bonierbale, Merideth W.
,
Tohme, Joseph M.
,
Verdier, Valérie
,
[Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)]
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Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)
The extent and structure of the genetic variability of traditional varieties of cassava (Manihot esculenta Crantz) have been little documented, despite considerable evidence for this crop's great varietal diversity in traditional agroecosystems. We used microsatellite markers to assess the geneti...
Elias, M.
,
Muhlen, GS
,
McKey, D
,
Roa, AC
,
Tohme, Joseph M.
,
[Genetic diversity of traditional South American landraces of cassava (Manihot esculenta Crantz): An analysis using microsatellites]
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Genetic diversity of traditional South American landraces of cassava (Manihot esculenta Crantz): An analysis using microsatellites
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationsh...
Álvarez, A.
,
Fuentes, JL
,
Puldon, V
,
Gómez, P.J.
,
Mora, L
,
Duque E., Myriam Cristina
,
Gallego Sánchez, Gerardo J.
,
Tohme, Joseph M.
,
[Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers]
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Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers