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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease m...


Restrepo, S.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis]AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis
González Torres, Rosa InésGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA]Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

Background Cassava, an allotetraploid known for its remarkable tolerance to abiotic stresses is an important source of energy for humans and animals and a raw material for many industrial processes. A full-length cDNA library of cassava plants under normal, heat, drought, aluminum and post harves...


Sakurai, TetsuyaPlata, GermanRodriguez, FaustoSeki, MotoaliSalcedo, AndresToyoda, AtsushiHiwata, AtsushiTohme, Joseph M.Sake, YoshiyukiShinozaki, KazuoIshitani, Manabu[Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response]Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response

Cassava is a tropical storage-root crop that serves as a worldwide source of staple food for over 800 million people. Flowering is one of the most important breeding challenges in cassava because in most lines flowering is late and non-synchronized, and flower production is sparse. The FLOWERING ...


Adeyemo, O. SarahChavarriaga Aguirre, PaulTohme, Joseph M.Fregene, Martin A.Davis, Seth J.Setter, Tim L.[Overexpression of Arabidopsis FLOWERING LOCUS T (FT) gene improves floral development in cassava (Manihot esculenta, Crantz)]Overexpression of Arabidopsis FLOWERING LOCUS T (FT) gene improves floral development in cassava (Manihot esculenta, Crantz)

Storage roots of cassava (Manihot esculenta Crantz) exhibit a rapid post-harvest physiological deterioration (PPD) response that can occur within 24 72 h of harvest. PPD is an enzymatically mediated oxidative process with parallels to plant wound, senescence and defence responses. To characterise...


Reilly, KBernal Franco, D.M.Cortés Bermúdez, D.F.Gómez Vásquez, RTohme, Joseph M.Beeching, JR[Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration]Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration

Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...


Sánchez, I.Angel, F.Grum, M.Duque E., Myriam CristinaLobo Arias, MTohme, Joseph M.Roca, William M.[Variability of chloroplast DNA in the genus Passiflora L.]Variability of chloroplast DNA in the genus Passiflora L.

Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...


Ramírez, H.Mayer, Jorge EdgardRoca, William M.Tohme, Joseph M.López, Y.[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético

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