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González Torres, Rosa InésGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA]Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA
Roca, William M.Angel, FernandoSarria, Rodrigo A.Mayer, Jorge EdgardTohme, Joseph M.Mejia, A.Mafla, G.[Future initiatives in biotechnology research for tropical agriculture: the case of cassava]Future initiatives in biotechnology research for tropical agriculture: the case of cassava

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...


Suarez, MCBernal, A.Gutiérrez, J.Tohme, Joseph M.Fregene, Martin A.[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

Background: Developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. Chromosome segment substitution lines from interspecific crosses represent a powerful an...


Gutiérrez, A.G.Carabalí, Silvio J.Giraldo Pasmín, O.X.Martínez Racines, César PompilioCorrea Victoria, Fernando J.Prado Patiño, G.A.Tohme, Joseph M.Lorieux, M[Identification of a rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa x O. glaberrima introgression lines]Identification of a rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa x O. glaberrima introgression lines

The angiosperm circadian clock has been well established from molecular-genetic studies in a temperate plant model. Conservation of clock function is less explored in plants from the tropics. Cassava (Manihot esculenta) is a staple crop grown in the tropics that has been of limited research inter...


Adeyemo, O. SarahKolmos, ETohme, Joseph M.Chavarriaga Aguirre, PaulFregene, Martin A.Davis, S.J.[Identification and characterization of the Cassava core-clock gene Early Flowering 4]Identification and characterization of the Cassava core-clock gene Early Flowering 4

More than 60% of common bean production worldwide is derived from cultivars of Middle American origin. Understanding the diversity of these will facilitate their use in genetic improvement. The objective of this study was to analyze a collection of 269 landraces of common bean (Phaseolus vulgaris...


Beebe, Stephen E.Skroch, PWTohme, Joseph M.Duque E., Myriam CristinaPedraza García, FabioNienhuis, James[Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD]Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD

A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained ...


López, C.E.Acosta, I.F.Jara, Carlos E.Pedraza García, FabioGaitán Solís, ElianaGallego Sánchez, Gerardo J.Beebe, Stephen E.Tohme, Joseph M.[Identifying resistance gene analogs associated with resistances to different pathogens in common beans]Identifying resistance gene analogs associated with resistances to different pathogens in common beans

Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils...


Anderson, J.W.Delseny, MFregene, Martin A.Jorge, VMba, CLópez, C.E.Restrepo, S.Soto Suárez, M.Piegu, BVerdier, ValérieCooke, RGTohme, Joseph M.Horvat, DP[An EST resource for cassava and other species of Euphorbiaceae]An EST resource for cassava and other species of Euphorbiaceae

DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia...


Segura, Sergio D.Coppens d'Eeckenbrugge, GeoBohórquez Cháux, AdrianaOllitrault, PTohme, Joseph M.[An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia]An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia

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