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The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (...


Caicedo, ALGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America]AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America

Number of spikelets per panicle (NSP) is a key trait to increase yield potential in rice (O. sativa). The architecture of the rice inflorescence which is mainly determined by the length and number of primary (PBL and PBN) and secondary (SBL and SBN) branches can influence NSP. Although several ge...


Rebolledo, María CamilaPeña, Alexandra L.Duitama, JorgeCruz, Daniel FelipeGrenier, CécileTohme, Joseph M.[Combining image analysis,genome wide association studies and different field trials to reveal stable genetic regions related to panicle architecture and the number of spikelets per panicle in rice]Combining image analysis,genome wide association studies and different field trials to reveal stable genetic regions related to panicle architecture and the number of spikelets per panicle in rice

Apomixis, asexual reproduction through seed, enables breeders to identify and faithfully propagate superior heterozygous genotypes by seed without the disadvantages of vegetative propagation or the expense and complexity of hybrid seed production. The availability of new tools such as genotyping ...


Worthington, MargaretHeffelfinger, ChristopherBernal, DianaQuintero, ConstanzaZapata, Yeny PatriciaPérez, Juan GuillermoVega, Jose deMiles, John W.Dellaporta, Stephen L.Tohme, Joseph M.[A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens]A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens
Rojas, MarcelaBeebe, Stephen E.Pedraza García, FabioMuñoz Arboleda, FernandoBeeck, Douglas P.Lynch, Jonathan P.Snapp, Sieglinde S.Tohme, Joseph M.[Mapping of genes for root characters in common bean]Mapping of genes for root characters in common bean

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

Low soil P availability is a primary constraint to common bean (Phaseolus vulgaris L.) production in Latin America and Africa. Substantial genotypic variation in bean adaptation to low phosphorus (LP) availability has been linked with root traits that enhance the efficiency of soil foraging. The ...


Beebe, Stephen E.Rojas, MYan, XBlair, Matthew W.Pedraza García, FabioMuñoz Arboleda, FernandoTohme, Joseph M.Lynch, Jonathan P.[Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean]Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean

Single nucleotide polymorphism (SNP) markers are by far the most common form of DNA polymorphism in a genome. The objectives of this study were to discover SNPs in common bean (Phaseolus vulgaris L.) by comparing sequences from coding and noncoding regions obtained from the GenBank and genomic DN...


Gaitán Solís, ElianaChoi, I.Y.Quigley, CCregan, P.B.Tohme, Joseph M.[Single nucleotide polymorphisms in common bean : Their discovery and genotyping using a multiplex detection system]Single nucleotide polymorphisms in common bean : Their discovery and genotyping using a multiplex detection system

Late root bulking is a major factor leading to rejection and abandoning of improved cassava genotypes in sub-Saharan Africa. Early bulking (EB) varieties shorten the growth period from planting to harvesting, better fit into environments with short rainy season, and reduce exposure to biotic and ...


Olasanmi, BAkoroda, M.O.Okogbenin, EmmanuelEgesi, Chiedozie N.Kahya, SSOnyegbule, OEwa, F.Gutiérrez, J.Ceballos, H.Tohme, Joseph M.Fregene, Martin A.[Bulked segregant analysis identifies molecular markers associated with early bulking in cassava (Manihot esculenta Crantz)]Bulked segregant analysis identifies molecular markers associated with early bulking in cassava (Manihot esculenta Crantz)

Recent advances in high-throughput sequencing (HTS) technologies and computing capacity have produced unprecedented amounts of genomic data that have unraveled the genetics of phenotypic variability in several species. However, operating and integrating current software tools for data analysis st...


Duitama, JorgeQuintero, Juan CamiloCruz, Daniel FelipeQuintero, ConstanzaHubmann, GeorgFoulquié Moreno, Maria R.Verstrepen, Kevin J.Trevelein, Johan MTohme, Joseph M.[An integrated framework for discovery and genotyping of genomic variants from high-throughput sequencing experiments]An integrated framework for discovery and genotyping of genomic variants from high-throughput sequencing experiments

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