African Cape buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (buffalo) has been r...
Bishop, Richard P.
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Hemmink, W.I.
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Morrison, J.D.
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Weir, W.
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Toye, Philip G.
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Sitt, T.
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Spooner, P.R.
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Musoke, A.J.
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Skilton, Robert A.
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Odongo, David O.
,
[The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes]
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The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Tick-borne diseases are a major impediment to improved productivity of livestock in sub-Saharan Africa. Improved control of these diseases would be assisted by detailed epidemiological data. Here we used longitudinal, serological data to determine the patterns of exposure to Theileria parva, Thei...
Kiara, Henry K.
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Jennings, A.
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Bronsvoort, B.M. de C.
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Hande, I.G.
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Mwangi, S.T.
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Mbole-Kariuki, Mary N.
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Wyk, I.C. van
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Poole, Elizabeth J.
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Hanotte, Olivier H.
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Coetzer, J.A.W.
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Woolhouse, Mark E.J.
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Toye, Philip G.
,
[A longitudinal assessment of the serological response to Theileria parva and other tick-borne parasites from birth to one year in a cohort of indigenous calves in western Kenya]
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A longitudinal assessment of the serological response to Theileria parva and other tick-borne parasites from birth to one year in a cohort of indigenous calves in western Kenya
Parasite burden varies widely between individuals within a population, and can covary with multiple aspects of individual phenotype. Here we investigate the sources of variation in faecal strongyle eggs counts, and its association with body weight and a suite of haematological measures, in a coho...
Callaby, R.
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Hanotte, Olivier H.
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Wyk, I.C. van
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Kiara, Henry K.
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Toye, Philip G.
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Mbole-Kariuki, Mary N.
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Jennings, A.
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Thumbi, S.M.
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Coetzer, J.A.W.
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Bronsvoort, B.M. de C.
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Knott, S.A.
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Woolhouse, Mark E.J.
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Kruuk, L.E.B.
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[Variation and covariation in strongyle infection in East African shorthorn zebu calves]
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Variation and covariation in strongyle infection in East African shorthorn zebu calves
We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture ...
Toye, Philip G.
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Teale, A.J.
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[Gene transfection in mammalian cells and its application in studies of immunity to East Coast fever]
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Gene transfection in mammalian cells and its application in studies of immunity to East Coast fever
Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...
Toye, Philip G.
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Gobright, E.I.
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Nyanjui, J.
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Nene, Vishvanath
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Bishop, Richard P.
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[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]
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Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva
Toye, Philip G.
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Goddeeris, M.
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Iams, K.P.
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Musoke, A.J.
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Morrison, W. Ivan
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[Characterization of a polymorphic immunodominant molecule in sporozoites and schizonts of Theileria parva]
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Characterization of a polymorphic immunodominant molecule in sporozoites and schizonts of Theileria parva
Most studies of infectious diseases in East African cattle have concentrated on gastro-intestinal parasites and vector-borne diseases. As a result, relatively little is known about viral diseases, except for those that are clinically symptomatic or which affect international trade such as foot an...
Callaby, R.
,
Toye, Philip G.
,
Jennings, A.
,
Thumbi, S.M.
,
Wyk, I.C. van
,
Hanotte, Olivier H.
,
Mbole-Kariuki, Mary N.
,
Bronsvoort, B.M. de C.
,
Kruuk, L.E.B.
,
Woolhouse, Mark E.J.
,
Kiara, Henry K.
,
[Seroprevalence of respiratory viral pathogens of indigenous calves in western Kenya]
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Seroprevalence of respiratory viral pathogens of indigenous calves in western Kenya
Peste des petits ruminants (PPR) is a major constraint to the livelihoods and food security of small scale farmers. The epidemiology and biology of PPR virus has much in common with rinderpest virus (RP), an agent that has been globally eradicated. This document presents a strategy for the progre...
Elsawalhy, A.
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Mariner, Jeffrey C.
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Chibeu, D.
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Wamway, H.
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Wakhusama, S.
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Olaho-Mukani, W.
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Toye, Philip G.
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[Pan African strategy for the progressive control of peste des petits ruminants (Pan African ppr strategy)]
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Pan African strategy for the progressive control of peste des petits ruminants (Pan African ppr strategy)
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
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Morzaria, S.P.
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Toye, Philip G.
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Skilton, Robert A.
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Nene, Vishvanath
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Nkonge, C.
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Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
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An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule