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Peste des petits ruminants (PPR) is a major constraint to the livelihoods and food security of small scale farmers. The epidemiology and biology of PPR virus has much in common with rinderpest virus (RP), an agent that has been globally eradicated. This document presents a strategy for the progre...


Elsawalhy, A.Mariner, Jeffrey C.Chibeu, D.Wamway, H.Wakhusama, S.Olaho-Mukani, W.Toye, Philip G.[Pan African strategy for the progressive control of peste des petits ruminants (Pan African ppr strategy)]Pan African strategy for the progressive control of peste des petits ruminants (Pan African ppr strategy)

For the November 2011 'liveSTOCK Exchange' event at the International Livestock Research Institute (ILRI), Phil Toye, reflects on ILRI's vaccine and diagnostics research accomplishments, from the past 10 years, that involved partnerships with other centres and countries in Africa.


Toye, Philip G.[ILRI research on animal diseases in Africa—Developing vaccines and diagnostics]ILRI research on animal diseases in Africa—Developing vaccines and diagnostics

In this presentation we would like to introduce a novel diagnostic tool for Taenia solium cysticercosis in the porcine host. This tool is a rapid, user-friendly lateral flow format assay utilising the HP10 monoclonal antibody. We will describe the production of this assay, its performance as judg...


Doble, L.F.Fèvre, Eric M.Bronsvoort, B.M. de C.Harrison, L.J.S.Parkhouse, R.M.E.Toye, Philip G.[Development of a rapid, user-friendly, diagnostic test for Taenia solium cysticercosis]Development of a rapid, user-friendly, diagnostic test for Taenia solium cysticercosis

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...


Katende, J.Morzaria, S.P.Toye, Philip G.Skilton, Robert A.Nene, VishvanathNkonge, C.Musoke, A.J.[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Sitt, T.Pelle, RogerWeir, W.Steinaa, LucillaShiels, B.Morrison, W. IvanToye, Philip G.[Theileria parva: Genotypic and antigenic diversity in buffalo and buffalo-associated cattle]Theileria parva: Genotypic and antigenic diversity in buffalo and buffalo-associated cattle

The polymorphic immunodominant molecule (PIM) of Theileria parva is expressed by the schizont and sporozoite stages of the parasite. We have recently cloned the cDNA encoding the PIM antigen from two stocks of the parasite: the cattle-derived T. parva (Muguga) stock and a buffalo-derived stock. T...


Toye, Philip G.Nyanjui, J.Goddeeris, B.M.Musoke, A.J.[Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva]Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva

Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...


Toye, Philip G.Metzelaar, M.J.Wijngaard, P.L.J.Nene, VishvanathIams, K.P.Roose, J.Nyanjui, J.K.Gobright, E.I.Musoke, A.J.Clevers, H.C.[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva
Hobson-Peters, J.Shan, J.Hall, R.A.Toye, Philip G.[Mammalian expression of functional autologous red blood cell agglutination reagents for use in diagnostic assays]Mammalian expression of functional autologous red blood cell agglutination reagents for use in diagnostic assays

The studies reported here describe methodology permiting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay....


Toye, Philip G.Wijngaard, P.L.J.MacHugh, Niall D.Clevers, H.[An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells]An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells
Goddeeris, B.M.Toye, Philip G.Morrison, W. Ivan[Strain specificity of bovine Theileria parva-specific cytotoxic T cells is determined by the phenotype of the restricting class I MHC]Strain specificity of bovine Theileria parva-specific cytotoxic T cells is determined by the phenotype of the restricting class I MHC

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